Supplementary Materials

Supplementary Materials for:

Pheromone-Induced Morphogenesis Improves Osmoadaptation Capacity by Activating the HOG MAPK Pathway

Rodrigo Baltanás, Alan Bush, Alicia Couto, Lucía Durrieu, Stefan Hohmann, Alejandro Colman-Lerner*

*Corresponding author. E-mail:

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  • Fig. S1. Behavior of the constitutive control reporter in cells adapted to high external osmolarity and comparison of Hog1 phosphorylation in cells acutely shocked and cells adapted to high osmotic strength media.
  • Fig. S2. Quantification criteria for Hog1 subcellular localization.
  • Fig. S3. Hog1-Venus nuclear localization at different external osmolarities in single cells.
  • Fig. S4. PR outputs during HOG activation induced by α factor.
  • Fig. S5. Δhog1 cells do not induce PSTL1-YFP in response to pheromone.
  • Fig. S6. Fus3 is necessary for the pheromone-induced activation of HOG.
  • Fig. S7. HOG transcriptional output is induced during mating in high external osmolarity.
  • Fig. S8. Large cell-to-cell variability in the pheromone-dependent activation of HOG.
  • Fig. S9. Alternative ordering of cells on the basis of HOG transcriptional bursts and the timing of shmoo formation.
  • Fig. S10. Correlation between shmooing and HOG output during pheromone-dependent HOG activation.
  • Fig. S11. The effect of the cell cycle position on the timing of HOG activation by pheromone.
  • Fig. S12. Mating projection classification and their distribution in response to different pheromone concentrations.
  • Fig. S13. Examples of mating projection classification at different pheromone concentrations.
  • Fig. S14. Quantification of MAPK phosphorylation in wild-type and mutant strains in response to pheromone.
  • Fig. S15. Additional data from the extracellular glycerol accumulation experiments.
  • Fig. S16. HOG reporter activation in wild-type and mutant strains in response to an acute hyperosmotic shock.
  • Fig. S17. Additional volume recovery experiments.
  • Table S1. Statistical analysis of phosphorylation of MAPKs related to fig. S14.
  • Table S2. Yeast strains.

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Citation: R. Baltanás, A. Bush, A. Couto, L. Durrieu, S. Hohmann, A. Colman-Lerner, Pheromone-Induced Morphogenesis Improves Osmoadaptation Capacity by Activating the HOG MAPK Pathway. Sci. Signal. 6, ra26 (2013).

© 2013 American Association for the Advancement of Science