Comparative Phosphoproteomic Analysis of Checkpoint Recovery Identifies New Regulators of the DNA Damage Response
Vincentius A. Halim, Mónica Alvarez-Fernández, Yan Juan Xu, Melinda Aprelia, Henk W. P. van den Toorn, Albert J. R. Heck, Shabaz Mohammed, René H. Medema*
*Corresponding author. E-mail: firstname.lastname@example.org
This PDF file includes:
- Fig. S1. Schematic of the checkpoint recovery assay.
- Fig. S2. In-depth proteomics data analysis.
- Fig. S3. Enrichment of candidates with particular functions.
- Fig. S4. Astrin affects the p53/MDM2 feedback loop.
- Legends for tables S1 to S8
Format: Adobe Acrobat PDF
Size: 345 KB
Other Supplementary Material for this manuscript includes the following:
- Table S1 (PDF and Excel format). All quantified phosphopeptides.
- Table S2 (PDF and Excel format). All identified peptides (unphosphorylated).
- Table S3 (PDF and Excel format). All identified proteins.
- Table S4 (PDF and Excel format). Comparing phosphosites that are phosphorylated by PLK1 and phosphosites of Polo box interactors.
- Table S5 (PDF and Excel format). Protein with changes on phosphopeptide abundance (at least in two experiments).
- Table S6 (PDF and Excel format). Comparing phosphosites that are phosphorylated by PLK1 during recovery and also phosphorylated with pSQ/TQ motif upon DNA damage.
- Table S7 (PDF and Excel format). Primary siRNA screen to identify proteins involved in recovery and unperturbed mitotic entry control.
- Table S8 (PDF and Excel format). Secondary screen for siRNA pool deconvolution.
Format: Adobe Acrobat PDF; Microsoft Excel
Size: 5.28 MB (PDF); 11.4 MB Excel unzipped
Update 26 April 2013
The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) through the PRIDE partner repository (1) with the dataset identifier PXD000222 and DOI 10.6019/PXD000222.
1. J. A. Vizcaíno, R. G. Côté, A. Csordas, J. A. Dianes, A. Fabregat, J. M. Foster, J. Griss, E. Alpi, M. Birim, J. Contell, G. O'Kelly, A. Schoenegger, D. Ovelleiro, Y. Pérez-Riverol, F. Reisinger, D. Ríos. R. Wang, H. Hermjakob. The Proteomics Identifications (PRIDE) database and associated tools: status in 2013. Nucleic Acids Res. 41,D1063-9 (2013).
Files are packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the instructions below.
Instructions for downloading and decompressing files:
Create a temporary folder on your machine's hard drive.
Save the compressed archive to the temporary folder you created, using the links above.
Citation: V. A. Halim, M. Alvarez-Fernández, Y. J. Xu, M. Aprelia, H. W. P. van den Toorn, A. J. R. Heck, S. Mohammed, R. H. Medema, Comparative Phosphoproteomic Analysis of Checkpoint Recovery Identifies New Regulators of the DNA Damage Response. Sci. Signal. 6, rs9 (2013).