Supplementary Materials

Supplementary Materials for:

Ubiquitin-Specific Protease 25 Regulates TLR4-Dependent Innate Immune Responses Through Deubiquitination of the Adaptor Protein TRAF3

Bo Zhong, Xikui Liu, Xiaohu Wang, Xindong Liu, Hongxiu Li, Bryant G. Darnay, Xin Lin, Shao-Cong Sun, Chen Dong*

*Corresponding author. E-mail: cdong@mdanderson.org

This PDF file includes:

  • Fig. S1. USP25 interacts with TRAF3.
  • Fig. S2. Deficiency in USP25 does not affect poly(I:C)-stimulated production of cytokines in vivo.
  • Fig. S3. USP25 does not regulate TLR3-dependent activation of NF-κB and MAPKs in MEFs.
  • Fig. S4. Role of USP25 in wild-type and Usp25−/− BMDMs in response to LPS or poly(I:C).
  • Fig. S5. Reconstitution of Usp25−/− MEFs with USP25 or USP25(C178S) has no effect on poly(I:C)-induced production of proinflammatory cytokines or type I IFNs.
  • Fig. S6. USP25 removes K48-linked ubiquitin chains from TRAF3(RM).
  • Fig. S7. Role of USP25 in the deubiquitination of TRAF6.

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Citation: B. Zhong, X. Liu, X. Wang, X. Liu, H. Li, B. G. Darnay, X. Lin, S.-C. Sun, C. Dong, Ubiquitin-Specific Protease 25 Regulates TLR4-Dependent Innate Immune Responses Through Deubiquitination of the Adaptor Protein TRAF3. Sci. Signal. 6, ra35 (2013).

© 2013 American Association for the Advancement of Science