Supplementary Materials

Supplementary Materials for:

Inhibition of TGF-β Signaling at the Nuclear Envelope: Characterization of Interactions Between MAN1, Smad2 and Smad3, and PPM1A

Benjamin Bourgeois, Bernard Gilquin, Carine Tellier-Lebègue, Cecilia Östlund, Wei Wu, Javier Pérez, Perla El Hage, François Lallemand, Howard J. Worman, Sophie Zinn-Justin*

*Corresponding author. E-mail: sophie.zinn{at}cea.fr

This PDF file includes:

  • Fig. S1. Yeast two-hybrid assay results for the interactions between the Smad2 single mutants and MAN1 730 to 910.
  • Fig. S2. Binding curve obtained by ITC for the FAST1 peptide and the phosphomimetic mutant of the MH2 domain of Smad2 (S2LMH2EEE).
  • Fig. S3. Evolution of the χ value as a function of the number of models used in the calculation of the average SAXS intensity.
  • Fig. S4. Sequence alignment of human Smad2 and Smad3 proteins.
  • Fig. S5. Replicate blots corresponding to Fig. 6, A and B.
  • Table S1. SAXS data collection and scattering-derived parameters.

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Citation: B. Bourgeois, B. Gilquin, C. Tellier-Lebègue, C. Östlund, W. Wu, J. Pérez, P. El Hage, F. Lallemand, H. J. Worman, S. Zinn-Justin, Inhibition of TGF-β Signaling at the Nuclear Envelope: Characterization of Interactions Between MAN1, Smad2 and Smad3, and PPM1A. Sci. Signal. 6, ra49 (2013).

© 2013 American Association for the Advancement of Science