Supplementary Materials

Sci. Signal., 3 September 2013
DOI: 10.1126/scisignal.2004496
Supplementary Materials for:

The Endoplasmic Reticulum Acts as a Platform for Ubiquitylated Components of Nuclear Factor κB Signaling

Catherine Alexia, Konstantinos Poalas, Gabrielle Carvalho, Naima Zemirli, Julie Dwyer, Sonia M. Dubois, Emeline M. Hatchi, Nelia Cordeiro, Sherri S. Smith, Céline Castanier, Armelle Le Guelte, Liling Wan, Yibin Kang, Aimé Vazquez, Julie Gavard, Damien Arnoult,* Nicolas Bidère*

*Corresponding author. E-mail: damien.arnoult@inserm.fr (D.A.); nicolas.bidere@inserm.fr (N.B.)

This PDF file includes:

  • Fig. S1. Characterization of the ubiquitylation of NF-κB signaling components.
  • Fig. S2. Ubiquitylated NF-κB signaling components accumulate in HM fractions in response to NOD2, TLR3, and TLR4 stimulation.
  • Fig. S3. Ubiquitylated NF-κB signaling components are found in HM fractions with minimal PM contamination.
  • Fig. S4. Activation of NF-κB in vitro by organelle-enriched cellular fractions.
  • Fig. S5. Identification of MTDH by mass spectrometry and analysis of its subcellular distribution.
  • Fig. S6. Participation of MTDH in cytokine-mediated activation of NF-κB.
  • Fig. S7. Effect of knockdown of MTDH on TCR-mediated early signaling and on IFN-β activity.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 2.40 MB


Citation: C. Alexia, K. Poalas, G. Carvalho, N. Zemirli, J. Dwyer, S. M. Dubois, E. M. Hatchi, N. Cordeiro, S. S. Smith, C. Castanier, A. Le Guelte, L. Wan, Y. Kang, A. Vazquez, J. Gavard, D. Arnoult, N. Bidère, The Endoplasmic Reticulum Acts as a Platform for Ubiquitylated Components of Nuclear Factor κB Signaling. Sci. Signal. 6, ra79 (2013).

© 2013 American Association for the Advancement of Science

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882