Supplementary Materials

Supplementary Materials for:

TRAF6 Stimulates the Tumor-Promoting Effects of TGFβ Type I Receptor Through Polyubiquitination and Activation of Presenilin 1

Shyam Kumar Gudey, Reshma Sundar, Yabing Mu, Anders Wallenius, Guangxiang Zang, Anders Bergh, Carl-Henrik Heldin, Marene Landström*

*Corresponding author. E-mail: marene.landstrom@medbio.umu.se

This PDF file includes:

  • Fig. S1. TGFβ promotes endoproteolytic cleavage of PS1.
  • Fig. S2. Schematic overview of the PLA.
  • Fig. S3. TGFβ promotes Lys63-dependent polyubiquitination of PS1 in cells.
  • Fig. S4. Identification of the PS1 cleavage site in TβRI.
  • Fig. S5. TGFβ promotes the colocalization of ectopically expressed TβRI and PS1, and treatment with monensin prevents activation of the γ-secretase complex and nuclear translocation of TβRI-ICD.
  • Fig. S6. TGFβ-induced invasive behavior of TRAMPC2 cells in vitro.
  • Fig. S7. Effects of a γ-secretase inhibitor on a xenograft prostate cancer model in vivo.
  • Fig. S8. Proposed simplified model of TGFβ-induced and TRAF6-dependent generation of TβRI-ICD.

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Citation: S. K. Gudey, R. Sundar, Y. Mu, A. Wallenius, G. Zang, A. Bergh, C.-H. Heldin, M. Landström, TRAF6 Stimulates the Tumor-Promoting Effects of TGFβ Type I Receptor Through Polyubiquitination and Activation of Presenilin 1. Sci. Signal. 7, ra2 (2014).

© 2014 American Association for the Advancement of Science