Supplementary Materials

Supplementary Materials for:

Roquin-2 Promotes Ubiquitin-Mediated Degradation of ASK1 to Regulate Stress Responses

Takeshi Maruyama, Toshihiro Araki, Yosuke Kawarazaki, Isao Naguro, Susanne Heynen, Pedro Aza-Blanc, Ze'ev Ronai, Atsushi Matsuzawa, Hidenori Ichijo*

*Corresponding author. E-mail: ichijo@mol.f.u-tokyo.ac.jp

This PDF file includes:

  • Text
    Fig. S1. Experimental procedures for the detection of ASK1 degradation.
  • Fig. S2. Imaging-based system for the detection of ASK1 proteasomal degradation.
  • Fig. S3. Validation of candidate E3 ligases targeting ASK1 with independent siRNAs.
  • Fig. S4. Validation of candidate E3 ligases targeting ASK1 by Western blot.
  • Fig. S5. Characterization of candidate E3 ligases targeting ASK1.
  • Fig. S6. Proteasome-dependent ASK1 degradation.
  • Fig. S7. Ubiquitination of the C terminus of ASK1 by Roquin-2.
  • Fig. S8. Evaluation of involvement of related signaling molecules in pathogen resistance.
  • References (2729)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Primary screen results and materials. (Excel file)
  • Table S2. Secondary screen results and materials. (Excel file)

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Citation: T. Maruyama, T. Araki, Y. Kawarazaki, I. Naguro, S. Heynen, P. Aza-Blanc, Z. Ronai, A. Matsuzawa, H. Ichijo, Roquin-2 Promotes Ubiquitin-Mediated Degradation of ASK1 to Regulate Stress Responses. Sci. Signal. 7, ra8 (2014).

© 2014 American Association for the Advancement of Science