Supplementary Materials

Supplementary Materials for:

The Nutrient-Responsive Transcription Factor TFE3 Promotes Autophagy, Lysosomal Biogenesis, and Clearance of Cellular Debris

José A. Martina, Heba I. Diab, Li Lishu, Lim Jeong-A, Simona Patange, Nina Raben, Rosa Puertollano*

*Corresponding author. E-mail: puertolr@mail.nih.gov

This PDF file includes:

  • Fig. S1. The intracellular distribution of TFE3 is regulated by nutrient levels and mTORC1 activity.
  • Fig. S2. Rag-mediated recruitment of TFE3 to lysosomes is critical for its retention in the cytosol in fully fed cells.
  • Fig. S3. Schematic representation of the subcellular distribution of TFE3 under nutrient-rich or starvation conditions.
  • Fig. S4. The intracellular distribution of FLCN is regulated by nutrient availability.
  • Fig. S5. Analysis of TFE3 and TFEB expression in different cell types and tissues.
  • Fig. S6. HeLa cell viability is not affected by overexpression of TFEB, MITF1, or TFE3 proteins.
  • Table S1. Gene expression changes in autophagy genes in response to TFEB, MITF1, or TFE3 overexpression.

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Citation: J. A. Martina, H. I. Diab, L. Lishu, L. Jeong-A, S. Patange, N. Raben, R. Puertollano, The Nutrient-Responsive Transcription Factor TFE3 Promotes Autophagy, Lysosomal Biogenesis, and Clearance of Cellular Debris. Sci. Signal. 7, ra9 (2014).

© 2014 American Association for the Advancement of Science