Supplementary Materials

Supplementary Materials for:

Yeast Osmosensors Hkr1 and Msb2 Activate the Hog1 MAPK Cascade by Different Mechanisms

Keiichiro Tanaka, Kazuo Tatebayashi,* Akiko Nishimura, Katsuyoshi Yamamoto, Hui-Yu Yang, Haruo Saito*

*Corresponding author. E-mail: tategone@ims.u-tokyo.ac.jp (K.T.); h-saito@ims.u-tokyo.ac.jp (H.S.)

This PDF file includes:

  • Fig. S1. Mode 1 and mode 2 signaling mechanisms of Sho1 and the Msb2 osmosensor.
  • Fig. S2. Crosstalk activation of the MAPK Kss1 by osmostress requires Bem1.
  • Fig. S3. The extracellular HMH domain is essential for Hog1 activation by the constitutively active Hkr1ΔSTR.
  • Fig. S4. Ste20-Sho1 binding moderately contributes to Hog1 activation by osmostress.
  • Fig. S5. Expression of Bem1 deletion constructs.
  • Table S1. Yeast strains used in this study.

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Citation: K. Tanaka, K. Tatebayashi, A. Nishimura, K. Yamamoto, H.-Y. Yang, H. Saito, Yeast Osmosensors Hkr1 and Msb2 Activate the Hog1 MAPK Cascade by Different Mechanisms. Sci. Signal. 7, ra21 (2014).

© 2014 American Association for the Advancement of Science