Supplementary Materials

Supplementary Materials for:

Mechanochemotransduction During Cardiomyocyte Contraction Is Mediated by Localized Nitric Oxide Signaling

Zhong Jian, Huilan Han, Tieqiao Zhang, Jose Puglisi, Leighton T. Izu, John A. Shaw, Ekama Onofiok, Jeffery R. Erickson, Yi-Je Chen, Balazs Horvath, Rafael Shimkunas, Wenwu Xiao, Yuanpei Li, Tingrui Pan, James Chan, Tamas Banyasz, Jil C. Tardiff, Nipavan Chiamvimonvat, Donald M. Bers, Kit S. Lam, Ye Chen-Izu*

*Corresponding author. E-mail: ychenizu@ucdavis.edu

This PDF file includes:

  • Materials and Methods
  • Fig. S1. PVA–cross-linker system for embedding single cardiomyocytes in a 3D elastic matrix.
  • Fig. S2. PVA or cross-linker alone did not affect contraction.
  • Fig. S3. Mechanical stress effects on the systolic Ca2+ transient and contraction.
  • Fig. S4. Effect of inhibiting nNOS or eNOS on cardiomyocytes.
  • Fig. S5. Distribution of RyR, nNOS, and eNOS in a wild-type cardiomyocyte.
  • Fig. S6. NO-dependent activation of CaMKII.
  • Legends for movies S1 to S3

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Technical Details

Format: Adobe Acrobat PDF

Size: 1.21 MB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Cell-in-gel contraction.
  • Movie S2 (.avi format). 3D rendition of nNOS-RyR colocalization.
  • Movie S3 (.avi format). 3D rendition of eNOS-RyR distribution.

[Download Movies S1 to S3]


Citation: Z. Jian, H. Han, T. Zhang, J. Puglisi, L. T. Izu, J. A. Shaw, E. Onofiok, J. R. Erickson, Y.-J. Chen, B. Horvath, R. Shimkunas, W. Xiao, Y. Li, T. Pan, J. Chan, T. Banyasz, J. C. Tardiff, N. Chiamvimonvat, D. M. Bers, K. S. Lam, Y. Chen-Izu, Mechanochemotransduction During Cardiomyocyte Contraction Is Mediated by Localized Nitric Oxide Signaling. Sci. Signal. 7, ra27 (2014).

© 2014 American Association for the Advancement of Science