Supplementary Materials

Supplementary Materials for:

Direct Imaging of Intracellular Signaling Components That Regulate Bacterial Chemotaxis

Hajime Fukuoka, Takashi Sagawa, Yuichi Inoue, Hiroto Takahashi, Akihiko Ishijima*

*Corresponding author. E-mail: ishijima@tagen.tohoku.ac.jp

This PDF file includes:

  • Fig. S1. Immunoblotting of CheY-GFP in whole-cell extracts with an anti-GFP antibody.
  • Fig. S2. Fluorescence intensity and rotational direction of a motor composed of FliM-GFP.
  • Fig. S3. Estimation of fluorescence intensity at the rotational center of a tethered cell.
  • Fig. S4. Extraction of the fluorescence intensity profile during switching events and time adjustment of the extracted profile.
  • Table S1. Bacterial strains and plasmids.
  • Table S2. Number of CheY-GFP molecules bound to flagellar motor.
  • Legends for movies S1 to S9
  • References (36, 37)

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 409 KB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Bright-field and fluorescence imaging of a tethered cell producing CheY-GFP.
  • Movie S2 (.mov format). Fluorescence imaging of a tethered cell expressing CheYGFP and producing CW-locked mutant motor.
  • Movie S3 (.mov format). Fluorescence imaging of a tethered cell expressing CheYGFP, producing CW-locked mutant motor, and treated with attractant.
  • Movie S4 (.mov format). Fluorescence imaging of a tethered cell expressing CheYGFP, producing CW-locked mutant motor, and treated with repellent.
  • Movie S5 (.mov format). Fluorescence imaging of a tethered cell expressing CheYGFP and producing wild-type motor.
  • Movie S6 (.mov format). Fluorescence imaging of a tethered cell expressing CheYGFP, producing wild-type motor, and treated with attractant.
  • Movie S7 (.mov format). Fluorescence imaging of a tethered cell expressing CheYGFP, producing wild-type mutant motor, and treated with repellent.
  • Movie S8 (.mov format). Fluorescence imaging of a tethered cell expressing caCheY-GFP and producing wild-type motor.
  • Movie S9 (.mov format). Fluorescence imaging of a tethered cell expressing caCheY-GFP and producing CW-locked mutant motor.

[Download Movies S1 to S9]


Citation: H. Fukuoka, T. Sagawa, Y. Inoue, H. Takahashi, A. Ishijima, Direct Imaging of Intracellular Signaling Components That Regulate Bacterial Chemotaxis. Sci. Signal. 7, ra32 (2014).

© 2014 American Association for the Advancement of Science