Supplementary Materials

Supplementary Materials for:

Parasitic Worms Stimulate Host NADPH Oxidases to Produce Reactive Oxygen Species That Limit Plant Cell Death and Promote Infection

Shahid Siddique, Christiane Matera, Zoran S. Radakovic, M. Shamim Hasan, Philipp Gutbrod, Elzbieta Rozanska, Miroslaw Sobczak, Miguel Angel Torres, Florian M. W. Grundler*

*Corresponding author. E-mail: grundler@uni-bonn.de

This PDF file includes:

  • Fig. S1. Nematode infection assays in Rboh mutant plants.
  • Fig. S2. Nematode infection assays in 35S::RbohD/rbohD plants.
  • Fig. S3. Development and invasion of nematodes treated with DPI.
  • Fig. S4. Plant syncytium size in rbohD/F plants.
  • Fig. S5. ROS visualization in roots of DPI-treated Col-0 plants.
  • Fig. S6. Light microscopy of uninfected Rboh mutant plants in secondary growth and infected Rboh mutant plants 14 dai.
  • Fig. S7. Transmission electron microscopy of Rboh mutant plants 5 and 14 dai.
  • Fig. S8. Cell viability in nematode-infected and uninfected plants treated with DPI.
  • Fig. S9. Analysis of gene expression in uninfected roots.
  • Table S1. Expression of RbohD and RbohF in syncytia of 35S::RbohD plants.
  • Table S2. Nematode and syncytium size in DPI-treated Col-0 plants 14 dai.
  • Table S3. Nematode and syncytium size in Col-0 plants 14 dai with nematodes preincubated with DPI.
  • Table S4. Expression of PR genes in Col-0 and rbohD/F/sid2 uninfected roots.
  • Table S5. Primers sequences used in this study.

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UPDATE: 9 April 2014, a new Supplementary Materials PDF file was uploaded because of a problem with visualizing figure S6. The data are unchanged.


Citation: S. Siddique, C. Matera, Z. S. Radakovic, M. S. Hasan, P. Gutbrod, E. Rozanska, M. Sobczak, M. A. Torres, F. M. W. Grundler, Parasitic Worms Stimulate Host NADPH Oxidases to Produce Reactive Oxygen Species That Limit Plant Cell Death and Promote Infection. Sci. Signal. 7, ra33 (2014).

© 2014 American Association for the Advancement of Science