Supplementary Materials

Supplementary Materials for:

Nuclear Envelope Lamin-A Couples Actin Dynamics with Immunological Synapse Architecture and T Cell Activation

José M. González-Granado,* Carlos Silvestre-Roig, Vera Rocha-Perugini, Laia Trigueros-Motos, Danay Cibrián, Giulia Morlino, Marta Blanco-Berrocal, Fernando G. Osorio, José M. P. Freije, Carlos López-Otín, Francisco Sánchez-Madrid,* Vicente Andrés*

*Corresponding author. E-mail: jmagonzalez@cnic.es (J.M.G.-G.); fsanchez.hlpr@salud.madrid.org
(F.S.-M.); vandres@cnic.es (V.A.)

This PDF file includes:

  • Fig. S1. Schemes of the protocols used to culture human PBLs and mouse splenocytes to generate human T lymphoblasts, human DCs, and mouse T lymphoblasts.
  • Fig. S2. Analysis of lamin-A/C and lamin-B1 in immune cells.
  • Fig. S3. CD4+ T cells migrate to lymph nodes and have lamin-A/C in the hapteninduced CHS model.
  • Fig. S4. J77 cells stably expressing GFP–lamin-A display enhanced T cell activation.
  • Fig. S5. Lamin-A facilitates the interactions between J77 cells and APCs and modulates CD3 movement and clustering at the immunological synapse.
  • Fig. S6. Quantification of the numbers of CD45.1+ and CD45.2+CD4+ T cells in the blood of donor, recipient, and chimeric mice.
  • Legends for movies S1 and S2

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Technical Details

Format: Adobe Acrobat PDF

Size: 1.79 MB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). A-type lamins regulate several steps in the interaction between T cells and APCs.
  • Movie S2 (.avi format). Lamin-A regulates the movement of TCR-CD3 complexes in the plasma membrane.

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Citation: J. M. González-Granado, C. Silvestre-Roig, V. Rocha-Perugini, L. Trigueros-Motos, D. Cibrián, G. Morlino, M. Blanco-Berrocal, F. G. Osorio, J. M. P. Freije, C. López-Otín, F. Sánchez-Madrid, V. Andrés, Nuclear Envelope Lamin-A Couples Actin Dynamics with Immunological Synapse Architecture and T Cell Activation. Sci. Signal. 7, ra37 (2014).

© 2014 American Association for the Advancement of Science