Supplementary Materials

Supplementary Materials for:

Dynamic Reprogramming of Signaling Upon Met Inhibition Reveals a Mechanism of Drug Resistance in Gastric Cancer

Andrea Z. Lai, Sean Cory, Hong Zhao, Mathieu Gigoux, Anie Monast, Marie-Christine Guiot, Sidong Huang, Ali Tofigh, Crista Thompson, Monica Naujokas, Victoria A. Marcus, Nicholas Bertos, Bita Sehat, Rushika M. Perera, Emily S. Bell, Brent D. G. Page, Patrick T. Gunning, Lorenzo E. Ferri, Michael Hallett, Morag Park*

*Corresponding author. E-mail: morag.park@mcgill.ca

This PDF file includes:

  • Fig. S1. Met IHC staining ranges in intensity in gastroesophageal primary tumors and lymph node metastases.
  • Fig. S2. Met inhibitor decreases the number of soft agar colonies.
  • Fig. S3. HGF-induced IEGs decrease upon Met inhibition.
  • Fig. S4. DUSP4 and DUSP6 are induced upon growth factor stimulation.
  • Fig. S5. Late expression of genes after HGF stimulation and PHA inhibition indicates proteins involved in the abrogation of cell proliferation.
  • Fig. S6. FOXO3A expression and downstream genes are differentially regulated upon Met inhibition.
  • Fig. S7. STAT3-targeted genes are differentially expressed in gastric cancer cell lines upon Met inhibition.
  • Fig. S8. Not all MAPK pathways are dependent on Met kinase activity.
  • Fig. S9. E2F1-targeted genes are differentially expressed in gastric cancer cell lines upon Met inhibition.
  • Fig. S10. DUSP loss upon Met inhibition is MEK-dependent, and subsequent ERK reactivation promotes phosphorylation of downstream effectors.
  • Fig. S11. Met inhibitors crizotinib and EMD-1214063 recapitulate effects on signaling observed with PHA.
  • Fig. S12. Inhibition of STAT3 hinders cell proliferation.
  • Fig. S13. STAT3 phosphorylation is dependent on Met kinase activity.
  • Fig. S14. Met inhibition promotes variable cytotoxicity in gastric cancer cell lines.
  • Table S1. MET amplification in primary gastric tumors.

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Citation: A. Z. Lai, S. Cory, H. Zhao, M. Gigoux, A. Monast, M.-C. Guiot, S. Huang, A. Tofigh, C. Thompson, M. Naujokas, V. A. Marcus, N. Bertos, B. Sehat, R. M. Perera, E. S. Bell, B. D. G. Page, P. T. Gunning, L. E. Ferri, M. Hallett, M. Park, Dynamic Reprogramming of Signaling Upon Met Inhibition Reveals a Mechanism of Drug Resistance in Gastric Cancer. Sci. Signal. 7, ra38 (2014).

© 2014 American Association for the Advancement of Science