Supplementary Materials

Supplementary Materials for:

Proteome-Wide Identification of SUMO2 Modification Sites

Triin Tammsalu, Ivan Matic, Ellis G. Jaffray, Adel F. M. Ibrahim, Michael H. Tatham, Ronald T. Hay*

*Corresponding author. E-mail: r.t.hay{at}

This PDF file includes:

  • Fig. S1. Comparison of parental and 6His-SUMO2T90K–expressing HEK293 cells.
  • Fig. S2. Comparison of SUMO2 and SUMO2T90K in conjugation and deconjugation reactions with various substrates.
  • Fig. S3. Overlap of SUMO2T90K sites in replicate experiments.
  • Fig. S4. Overlap of sumoylated proteins with published protein level proteomic studies.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Sumoylation sites identified in SUMO2T90K-expressing cells.
  • Table S2 (Microsoft Excel format). diGly-modified peptides identified in SUMO2T90K-expressing cells.
  • Table S3 (Microsoft Excel format). Functional annotation and network analysis of SUMO2T90K-expressing cells.
  • SMfile1.PDF. MS2 spectra of multiply sumoylated peptides identified from SUMO2T90K-expressing cells.
  • SMfile2.PDF. MS2 spectra of branched sumoylated peptides identified from SUMO2-expressing cells.
  • SUMO1.FASTA. SUMO1 virtual branched peptide database.
  • SUMO2.FASTA. SUMO2 virtual branched peptide database.

Citation: T. Tammsalu, I. Matic, E. G. Jaffray, A. F. M. Ibrahim, M. H. Tatham, R. T. Hay, Proteome-Wide Identification of SUMO2 Modification Sites. Sci. Signal. 7, rs2 (2014).

© 2014 American Association for the Advancement of Science