Supplementary Materials

Supplementary Materials for:

Nongenomic Thyroid Hormone Signaling Occurs Through a Plasma Membrane–Localized Receptor

Hema Kalyanaraman, Raphaela Schwappacher, Jisha Joshua, Shunhui Zhuang, Brian T. Scott, Matthew Klos, Darren E. Casteel, John A. Frangos, Wolfgang Dillmann, Gerry R. Boss, Renate B. Pilz*

*Corresponding author. E-mail: rpilz{at}ucsd.edu

This PDF file includes:

  • Fig. S1. Nongenomic TH signaling requires a TRα isoform.
  • Fig. S2. Identification of previously uncharacterized TRa isoforms and their function in nongenomic TH signaling.
  • Fig. S3. Membrane association of p30 TRα1.
  • Fig. S4. ERK and Akt activation by TH requires NO-cGMP-PKG signaling as well as SHP-2, Src, and the integrin β3 subunit.
  • Fig. S5. TH promotes osteoblast proliferation and osteocyte survival through nongenomic signaling.
  • Fig. S6. cGMP improves bone formation and prevents osteocyte apoptosis in hypothyroid mice.
  • Table S1. Antibodies used in this study.
  • Table S2. siRNA target sequences.
  • Table S3. Primer sequences.

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Citation: H. Kalyanaraman, R. Schwappacher, J. Joshua, S. Zhuang, B. T. Scott, M. Klos, D. E. Casteel, J. A. Frangos, W. Dillmann, G. R. Boss, R. B. Pilz, Nongenomic Thyroid Hormone Signaling Occurs Through a Plasma Membrane–Localized Receptor. Sci. Signal. 7, ra48 (2014).

© 2014 American Association for the Advancement of Science