Supplementary Materials

Supplementary Materials for:

The Kinase LMTK3 Promotes Invasion in Breast Cancer Through GRB2-Mediated Induction of Integrin β1

Yichen Xu, Hua Zhang, Lei C. Lit, Arnhild Grothey, Maria Athanasiadou, Marianna Kiritsi, Ylenia Lombardo, Adam E. Frampton, Andrew R. Green, Ian O. Ellis, Simak Ali, Heinz-Josef Lenz, Maya Thanou, Justin Stebbing, Georgios Giamas*

*Corresponding author. E mail: g.giamas@imperial.ac.uk

This PDF file includes:

  • Fig. S1. MS analysis of LMTK3 protein bands.
  • Fig. S2. LMTK3 promotes migration in T47D cells.
  • Fig. S3. Gene Ontology classification of LMTK3-regulated proteins in MCF7 cells using DAVID Functional Annotation tools.
  • Fig. S4. LMTK3 promotes integrin expression.
  • Fig. S5. SRF is required for LMTK3-regulated cell migration but not for LMTK3-induced proliferation.
  • Fig. S6. LMTK3 promotes cancer cell proliferation through a GRB2-RAS-ERK/MAPK pathway and cell invasion through a GRB2-CDC42-SRF-integrin pathway.
  • Fig. S7. Integrin β1 abundance predicts clinical outcome in breast cancer patients.
  • Legend for table S1
  • Legend for movie S1

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Technical Details

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). SILAC analysis of LMTK3-silenced MCF7 cells.
  • Movie S1 (.wmv format). LMTK3 promotes MCF7 cell motility.

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Citation: Y. Xu, H. Zhang, L. C. Lit, A. Grothey, M. Athanasiadou, M. Kiritsi, Y. Lombardo, A. E. Frampton, A. R. Green, I. O. Ellis, S. Ali, H.-J. Lenz, M. Thanou, J. Stebbing, G. Giamas, The Kinase LMTK3 Promotes Invasion in Breast Cancer Through GRB2- Mediated Induction of Integrin β1. Sci. Signal. 7, ra58 (2014).

© 2014 American Association for the Advancement of Science