Supplementary Materials

Supplementary Materials for:

Exosomes from bone marrow mesenchymal stem cells contain a microRNA that promotes dormancy in metastatic breast cancer cells

Makiko Ono, Nobuyoshi Kosaka, Naoomi Tominaga, Yusuke Yoshioka, Fumitaka Takeshita, Ryou-u Takahashi, Masayuki Yoshida, Hitoshi Tsuda, Kenji Tamura, Takahiro Ochiya*

*Corresponding author. E-mail: tochiya@ncc.go.jp

This PDF file includes:

  • Fig. S1. Establishment of a line of breast cancer cells that metastasized to bone marrow.
  • Fig. S2. Gene expression array in BM2 and parental cells.
  • Fig. S3. Surface marker profiles of BM-MSCs from human donors.
  • Fig. S4. Flow cytometric analysis of PKH26 labeling in BM2 cultures.
  • Fig. S5. CD44 expression and surface abundance on sorted CD44+ and CD44 cells.
  • Fig. S6. Gene expression array in CD44+ or CD44 BM2 cells.
  • Fig. S7. Characterization of BM-MSC–derived exosomes.
  • Fig. S8. Images of BM2 cells cocultured with SYTO64-labeled BM-MSCs.
  • Fig. S9. Images of BM2 cells cocultured with PKH26-labeled exosomes.
  • Fig. S10. Flow cytometry of BM2 cells cultured with BM-MSC–derived exosomes.
  • Fig. S11. Proliferation and invasive behavior of BM2 cells cultured with BM-MSC–derived exosomes.
  • Fig. S12. Expression of miR-23b in exosomes derived from BM-MSCs or fibroblasts.
  • Fig. S13. Overexpression of miR-23b in BM2 cells.
  • Fig. S14. Proliferation and invasive behavior of BM2 cells overexpressing miR-23b.
  • Fig. S15. Effect of exosomes on miR-23b and MARCKS expression in BM2 cells.
  • Fig. S16. Knockdown of MARCKS in BM2 cells.
  • Fig. S17. Proliferation and invasive behavior in MARCKS-deficient BM2 cells.
  • Table S1. Donor information for BM-MSCs.
  • Table S2. miRNAs that were increased in BM-MSC exosomes.
  • Table S3. Predicted miR-23b target genes.
  • Legend for table S4

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Other Supplementary Material for this manuscript includes the following:

  • Table S4 (Microsoft Excel format). Expression analysis in BM2 cells.

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Citation: M. Ono, N. Kosaka, N. Tominaga, Y. Yoshioka, F. Takeshita, R.-u Takahashi, M. Yoshida, H. Tsuda, K. Tamura, T. Ochiya, Exosomes from bone marrow mesenchymal stem cells contain a microRNA that promotes dormancy in metastatic breast cancer cells. Sci. Signal. 7, ra63 (2014).

© 2014 American Association for the Advancement of Science