Supplementary Materials

Supplementary Materials for:

The autoinhibitory C-terminal SH2 domain of phospholipase C–γ2 stabilizes B cell receptor signalosome assembly

Jing Wang, Haewon Sohn, Guangping Sun, Joshua D. Milner, Susan K. Pierce*

*Corresponding author. E-mail: spierce{at}nih.gov

This PDF file includes:

  • Fig. S1. Less BCRs traffic into the HLA-DM+ class II–processing compartment upon BCR cross-linking in PLAID B cells than in HC donor B cells.
  • Legends for movies S1 to S3

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Technical Details

Format: Adobe Acrobat PDF

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Accumulation of BCRs in B cells expressing WT PLC-γ2–GFP or Δ20–22 PLC-γ2–GFP at the interface of the cell and anti-IgM lipid bilayers.
  • Movie S2 (.mov format). Analysis of the increase in size of BCR microclusters in B cells expressing either WT PLC-γ2–GFP or Δ20–22 PLC-γ2–GFP incubated on anti-IgM bilayers.
  • Movie S3 (.mov format). Accumulation of BCRs and PLC–γ2 in B cells expressing WT PLC-γ2–GFP or Δ20–22 PLC-γ2–GFP at the interface of the cell and the anti-IgM bilayer.

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Citation: J. Wang, H. Sohn, G. Sun, J. D. Milner, S. K. Pierce, The autoinhibitory C-terminal SH2 domain of phospholipase C–γ2 stabilizes B cell receptor signalosome assembly. Sci. Signal. 7, ra89 (2014).

© 2014 American Association for the Advancement of Science