Supplementary Materials

Supplementary Materials for:

Invasive breast carcinoma cells from patients exhibit MenaINV- and macrophage-dependent transendothelial migration

Jeanine Pignatelli,* Sumanta Goswami, Joan G. Jones, Thomas E. Rohan, Evan Pieri, Xiaoming Chen, Esther Adler, Dianne Cox, Sara Maleki, Anne Bresnick, Frank B. Gertler, John S. Condeelis,* Maja H. Oktay*

*Corresponding author. E-mail: moktay{at}montefiore.org (M.H.O.); jeanine.pignatelli{at}einstein.yu.edu (J.P.); john.condeelis{at}einstein.yu.edu (J.S.C.)

This PDF file includes:

  • Fig. S1. The experimental setup of the iTEM assay and the permeability of HMEC endothelium.
  • Fig. S2. TMEM score and relative MenaINV abundance in LN-positive or LN-negative cases.
  • Fig. S3. iTEM assays engineered with HMEC-1 endothelium performed with human IDC cells obtained by FNA.
  • Table S1. MENA and GAPDH primer sequences.
  • Table S2. Correlation coefficients for TMEM score and relative expression of MENA isoforms in IDCs, overall and by clinical subtype.
  • Table S3. TMEM scores and relative Mena isoform expression for tumors with different clinical and pathological variables.
  • Table S4. Clinical and pathological data pertaining to iTEM data in fig. S3A.
  • Table S5. Clinical and pathological data pertaining to iTEM data in Figs. 4 and 6.

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Citation: J. Pignatelli, S. Goswami, J. G. Jones, T. E. Rohan, E. Pieri, X. Chen, E. Adler, D. Cox, S. Maleki, A. Bresnick, F. B. Gertler, J. S. Condeelis, M. H. Oktay, Invasive breast carcinoma cells from patients exhibit MenaINV- and macrophage-dependent transendothelial migration. Sci. Signal. 7, ra112 (2014).

© 2014 American Association for the Advancement of Science