Supplementary Materials

Supplementary Materials for:

HDL-bound sphingosine 1-phosphate acts as a biased agonist for the endothelial cell receptor S1P1 to limit vascular inflammation

Sylvain Galvani, Marie Sanson, Victoria A. Blaho, Steven L. Swendeman, Hideru Obinata, Heather Conger, Björn Dahlbäck, Mari Kono, Richard L. Proia, Jonathan D. Smith, Timothy Hla*

*Corresponding author. E-mail: tih2002{at}med.cornell.edu

This PDF file includes:

  • Fig. S1. S1P1 localization in en face preparations of the mouse aorta.
  • Fig. S2. ICAM-1 and VCAM-1 abundance in aorta upon S1P1 deletion.
  • Fig. S3. Effect of albumin and albumin-S1P on TNFα-induced increases in ICAM-1 and VCAM-1 abundance.
  • Fig. S4. Characterization of the sphingolipid composition of human HDL.
  • Fig. S5. Effect of S1P carrier on S1P1 retention at the cell surface.
  • Fig. S6. Effect of S1P1 inhibition in S1P-induced activation of MAPK.
  • Fig. S7. Subcellular localization of β-arrestin 2 depends on the S1P carrier.
  • Fig. S8. Genetic modulation of S1P1 expression in endothelial cells and modification of ICAM-1 abundance.
  • Fig. S9. Analysis of weight, plasma cholesterol, and cell populations in Apoe−/− S1pr1 wild-type and ECKO animals after 16 weeks on a high-fat diet.
  • Fig. S10. Plasma sphingolipid characterization.
  • Fig. S11. Analysis of plaque composition.
  • Fig. S12. Atherosclerotic root lesion quantification.

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Citation: S. Galvani, M. Sanson, V. A. Blaho, S. L. Swendeman, H. Conger, B. Dahlbäck, M. Kono, R. L. Proia, J. D. Smith, T. Hla, HDL-bound sphingosine 1-phosphate acts as a biased agonist for the endothelial cell receptor S1P1 to limit vascular inflammation. Sci. Signal. 8, ra79 (2015).

© 2015 American Association for the Advancement of Science