Supplementary Materials

Supplementary Materials for:

Purinergic P2Y6 receptors heterodimerize with angiotensin AT1 receptors to promote angiotensin II–induced hypertension

Akiyuki Nishimura, Caroline Sunggip, Hidetoshi Tozaki-Saitoh, Tsukasa Shimauchi, Takuro Numaga-Tomita, Katsuya Hirano, Tomomi Ide, Jean-Marie Boeynaems, Hitoshi Kurose, Makoto Tsuda, Bernard Robaye, Kazuhide Inoue, Motohiro Nishida*

*Corresponding author. E-mail: nishida{at}nips.ac.jp

This PDF file includes:

  • Fig. S1. Involvement of βArr2 in Ang II–induced AT1R internalization.
  • Fig. S2. Effects of agonists and antagonists on the heterodimerization of AT1R and P2Y6R.
  • Fig. S3. P2Y6R activation by Up4A.
  • Fig. S4. Ang II–induced activation of various signaling pathways in VSMCs from different developmental stages.
  • Fig. S5. Ang II induces Akt activation through Gq in VSMCs.
  • Fig. S6. Involvement of βArr in Ang II–mediated hyperplastic and hypertrophic signaling in VSMCs.
  • Fig. S7. Ang II promotes contractile differentiation of adult VSMCs.
  • Fig. S8. P2Y6R deletion inhibits Ang II–induced hypertrophic growth.
  • Fig. S9. Developmental increases in P2Y6R abundance enhance Ang II–induced hypertrophic signaling.
  • Fig. S10. P2Y6R deletion potentiates βArr-dependent signaling.
  • Fig. S11. MRS2578 attenuates Ang II–induced RhoA activation.
  • Table S1. List of siRNAs.
  • Table S2. List of Taqman probe and primer sets for real-time PCR.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 982 KB


Citation: A. Nishimura, C. Sunggip, H. Tozaki-Saitoh, T. Shimauchi, T. Numaga-Tomita, K. Hirano, T. Ide, J.-M. Boeynaems, H. Kurose, M. Tsuda, B. Robaye, K. Inoue, M. Nishida, Purinergic P2Y6 receptors heterodimerize with angiotensin AT1 receptors to promote angiotensin II–induced hypertension. Sci. Signal. 9, ra7 (2016).

© 2016 American Association for the Advancement of Science