Supplementary Materials

Supplementary Materials for:

The FOXM1 inhibitor RCM-1 suppresses goblet cell metaplasia and prevents IL-13 and STAT6 signaling in allergen-exposed mice

Lifeng Sun, Xiaomeng Ren, I-Ching Wang, Arun Pradhan, Yufang Zhang, Hannah M. Flood, Bo Han, Jeffrey A. Whitsett, Tanya V. Kalin, Vladimir V. Kalinichenko*

*Corresponding author. Email: vladimir.kalinichenko{at}cchmc.org

This PDF file includes:

  • Fig. S1. RCM-1 decreases FOXM1 abundance in U2OS cells and pulmonary airways.
  • Fig. S2. Lack of toxicity after RCM-1 treatment.
  • Fig. S3. RCM-1 protects bronchiolar epithelial cells from HDM-mediated effects.
  • Fig. S4. Identification of inflammatory cells in BALF of HDM-treated mice.
  • Fig. S5. RCM-1 protects bronchiolar epithelial cells from IL-13–mediated decreases in the abundance of FOXA2.
  • Fig. S6. Lung mechanics in mice treated with IL-13 and RCM-1.
  • Fig. S7. Lack of toxicity of RCM-1 in IL-13–treated mice.
  • Fig. S8. RCM-1 inhibits nuclear accumulation of FOXM1 protein in A549 cells.
  • Fig. S9. U0126 decreases lung inflammation but does not affect goblet cell metaplasia or the abundance of FOXM1.
  • Table S1. TaqMan assays for qRT-PCR.
  • Table S2. Histological evaluation of inflammatory responses in lung tissue.
  • Table S3. Histology scores of lung tissue from HDM-treated mice.

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Citation: L. Sun, X. Ren, I.-C. Wang, A. Pradhan, Y. Zhang, H. M. Flood, B. Han, J. A. Whitsett, T. V. Kalin, V. V. Kalinichenko, The FOXM1 inhibitor RCM-1 suppresses goblet cell metaplasia and prevents IL-13 and STAT6 signaling in allergen-exposed mice. Sci. Signal. 10, eaai8583 (2017).

© 2017 American Association for the Advancement of Science