Supplementary Materials

Supplementary Materials for:

Multiepitope tissue analysis reveals SPPL3-mediated ADAM10 activation as a key step in the transformation of melanocytes

Christian Ostalecki, Jung-Hyun Lee, Jochen Dindorf, Lena Collenburg, Stephan Schierer, Beate Simon, Stefan Schliep, Elisabeth Kremmer, Gerold Schuler, Andreas S. Baur*

*Corresponding author. Email: andreas.baur{at}uk-erlangen.de

This PDF file includes:

  • Fig. S1. Screening algorithm to obtain melanoma-specific antibodies.
  • Fig. S2. BRAFV600E staining of melanoma tissue.
  • Fig. S3. Assessment of relative protein expression levels after MELC analysis.
  • Fig. S4. Melanoma- but not nevi-associated keratinocytes have an altered PEP, and an enhanced fluorescence signal reveals nuclear Notch1 staining.
  • Fig. S5. Tissue sections of different nevi and SSM growth phases analyzed by MELC.
  • Fig. S6. Quantification of the keratinocyte PEP in healthy skin, different nevi, and SSM and coimmunoprecipitation of ADAM10 with SPPL3.
  • Fig. S7. Expression of SPPL3, SPPL2a, and SPPL2b in keratinocytes associated with healthy skin, nevi, and SSM.
  • Fig. S8. Coimmunoprecipitation of the SPPL3 but not SPPL2a or SPPL2b with ADAM10 and subcellular localization of SPPL3 in melanoma cells.
  • Fig. S9. Abundance of PTEN in SSM, healthy skin, and a dysplastic nevus.
  • Fig. S10. Expression control of adenoviral constructs in primary melanocytes.
  • Fig. S11. BRAFV600E and PTEN modulate ADAM10 activity in melanoma cells.
  • Fig. S12. Hypothetical model on the activation and role of ADAM10 in melanocyte transformation.
  • Table S1. List of antibodies used for MELC analysis.
  • Table S2. Melanoma-associated keratinocytes change their PEP.

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Citation: C. Ostalecki, J.-H. Lee, J. Dindorf, L. Collenburg, S. Schierer, B. Simon, S. Schliep, E. Kremmer, G. Schuler, A. S. Baur, Multiepitope tissue analysis reveals SPPL3-mediated ADAM10 activation as a key step in the transformation of melanocytes. Sci. Signal. 10, eaai8288 (2017).

© 2017 American Association for the Advancement of Science