Supplementary Materials

Supplementary Materials for:

A cluster of immunoresolvents links coagulation to innate host defense in human blood

Paul C. Norris, Stephania Libreros, Nan Chiang, Charles N. Serhan*

*Corresponding author. Email: cserhan{at}bwh.harvard.edu

This PDF file includes:

  • Fig. S1. SPMs and their biosynthetic pathways.
  • Fig. S2. LM identification in coagulated human peripheral blood.
  • Fig. S3. Coagulation leads to substantial increases in the production of SPMs, prostaglandins, and leukotrienes.
  • Fig. S4. Removal of adenosine enhances SPM production and platelet-neutrophil interactions during coagulation.
  • Fig. S5. SPM formation in human blood clots is regulated by lipoxygenase activity.
  • Fig. S6. Coagulation increases SPM production in hemorrhagic exudates.
  • Fig. S7. SPMs stimulate rapid increases in the abundances of intracellular phosphoproteins.
  • Fig. S8. SPMs induce intracellular signaling in monocytes and B cells in human whole blood.
  • Fig. S9. The inhibitor of SPM formation BAI reduces bacterial killing.
  • Fig. S10. LM profiles are distinct between normal and diseased human tissues.
  • Table S1. Abbreviations of LMs and SPMs.
  • Table S2. Leukocyte and lymphocyte populations are consistent during coagulation.
  • Table S3. Leukocytes and lymphocytes remain viable during coagulation.
  • Table S4. LM concentrations in human whole blood during coagulation.
  • Table S5. Leukocytes and lymphocytes are viable in the presence of eptifibatide or ADA during coagulation.
  • Table S6. LM concentrations in human whole blood during coagulation in the presence of indomethacin.
  • Table S7. Mass cytometry human blood antibody panel.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 7.09 MB


Citation: P. C. Norris, S. Libreros, N. Chiang, C. N. Serhan, A cluster of immunoresolvents links coagulation to innate host defense in human blood. Sci. Signal. 10, eaan1471 (2017).

© 2017 American Association for the Advancement of Science