Supplementary Materials

Supplementary Materials for:

A systems approach for discovering linoleic acid derivatives that potentially mediate pain and itch

Christopher E. Ramsden,* Anthony F. Domenichiello, Zhi-Xin Yuan, Matthew R. Sapio, Gregory S. Keyes, Santosh K. Mishra, Jacklyn R. Gross, Sharon Majchrzak-Hong, Daisy Zamora, Mark S. Horowitz, John M. Davis, Alexander V. Sorokin, Amit Dey, Danielle M. LaPaglia, Joshua J. Wheeler, Michael R. Vasko, Nehal N. Mehta, Andrew J. Mannes, Michael J. Iadarola

*Corresponding author. Email: chris.ramsden{at}nih.gov

This PDF file includes:

  • Fig. S1. Proton NMR data for authentic standards.
  • Fig. S2. Identification of hydroxy-epoxy-octadecenoate and keto-epoxy-octadecenoates.
  • Fig. S3. Basal and low pH–evoked CGRP release in response to lipid mediators.
  • Table S1. Rat tissue concentrations of hydroxy-epoxy- and keto-epoxy-octadecenoates.
  • Table S2. Concentrations of hydroxy-epoxy- and keto-epoxy-octadecenoates in control skin and psoriatic skin.
  • Table S3. Percentage of total hydroxy-epoxy- and keto-epoxy-octadecenoates as free acids in control skin and psoriatic skin.
  • Table S4. Serum concentrations of free hydroxy-epoxy- and keto-epoxy-octadecenoates in control and psoriatic patients.
  • Table S5. Association between diet-induced changes in plasma linoleic acid derivatives and pain-related end points in the CDH trial.
  • Table S6. Mass spectrometry parameters established for the sMRM mode.
  • Table S7. Demographic and clinical characteristics of psoriatic patients and controls.
  • Table S8. Baseline demographic and clinical characteristics of patients with CDH.

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Citation: C. E. Ramsden, A. F. Domenichiello, Z.-X. Yuan, M. R. Sapio, G. S. Keyes, S. K. Mishra, J. R. Gross, S. Majchrzak-Hong, D. Zamora, M. S. Horowitz, J. M. Davis, A. V. Sorokin, A. Dey, D. M. LaPaglia, J. J. Wheeler, M. R. Vasko, N. N. Mehta, A. J. Mannes, M. J. Iadarola, A systems approach for discovering linoleic acid derivatives that potentially mediate pain and itch. Sci. Signal. 10, eaal5241 (2017).

© 2017 American Association for the Advancement of Science