Supplementary Materials

Supplementary Materials for:

Hedgehog reciprocally controls trafficking of Smo and Ptc through the Smurf family of E3 ubiquitin ligases

Shuang Li, Shuangxi Li, Bing Wang, Jin Jiang*

*Corresponding author. Email: jin.jiang{at}utsouthwestern.edu

This PDF file includes:

  • Fig. S1. Cul4 acts in parallel with Smurf to promote Smo ubiquitylation and cell surface clearance.
  • Fig. S2. Effect of Smurf family knockdown on Smo cell surface accumulation and Hh signaling in wing discs.
  • Fig. S3. Effect of overexpression of Smurf family members on Smo cell surface accumulation and Hh signaling in wing discs.
  • Fig. S4. Hh-stimulated and PKA-mediated phosphorylation of Smo inhibit the recruitment of Su(dx) and Nedd4.
  • Fig. S5. Hh regulates Ptc ubiquitylation through the Smurf family of E3s.
  • Fig. S6. Regulation of Ptc-E3 interaction by Hh and Smo.
  • Fig. S7. Ptc interacts with Smurf regardless of Smurf phosphorylation by Gprk2.

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Citation: S. Li, S. Li, B. Wang, J. Jiang, Hedgehog reciprocally controls trafficking of Smo and Ptc through the Smurf family of E3 ubiquitin ligases. Sci. Signal. 11, eaan8660 (2018).

© 2018 American Association for the Advancement of Science