Error message

No crossref credentials set for plantcell

The Arabidopsis Cupin Domain Protein AtPirin1 Interacts with the G Protein α-Subunit GPA1 and Regulates Seed Germination and Early Seedling Development

Plant Cell, 1 July 2003
Vol. 15, Issue 7, p. 1578-1590
DOI: 10.1105/tpc.011890

The Arabidopsis Cupin Domain Protein AtPirin1 Interacts with the G Protein α-Subunit GPA1 and Regulates Seed Germination and Early Seedling Development

  1. Yevgeniya R. Lapik and
  2. Lon S. Kaufman1
  1. Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, Chicago, Illinois 60607
  1. 1To whom correspondence should be addressed. E-mail lkaufman{at}; fax 312-413-2691


Heterotrimeric G proteins are implicated in diverse signaling processes in plants, but the molecular mechanisms of their function are largely unknown. Finding G protein effectors and regulatory proteins can help in understanding the roles of these signal transduction proteins in plants. A yeast two-hybrid screen was performed to search for proteins that interact with Arabidopsis G protein α-subunit (GPA1). One of the identified GPA1-interacting proteins is the cupin-domain protein AtPirin1. Pirin is a recently defined protein found because of its ability to interact with a CCAAT box binding transcription factor. The GPA1–AtPirin1 interaction was confirmed in an in vitro binding assay. We characterized two atpirin1 T-DNA insertional mutants and established that they display a set of phenotypes similar to those of gpa1 mutants, including reduced germination levels in the absence of stratification and an abscisic acid–imposed delay in germination and early seedling development. These data indicate that AtPirin1 likely functions immediately downstream of GPA1 in regulating seed germination and early seedling development.

  • Received March 14, 2003.
  • Accepted April 28, 2003.
  • Published June 5, 2003.


Y. R. Lapik and L. S. Kaufman, The Arabidopsis Cupin Domain Protein AtPirin1 Interacts with the G Protein α-Subunit GPA1 and Regulates Seed Germination and Early Seedling Development. Plant Cell 15, 1578-1590 (2003).

Transcriptomics exposes the uniqueness of parasitic plants
Y. Ichihashi, J. M. Mutuku, S. Yoshida, and K. Shirasu
Briefings in Functional Genomics 0, elv001v1-elv001 (19 February 2015)

Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis
K. Klopffleisch, N. Phan, K. Augustin, R. S. Bayne, K. S. Booker, J. R. Botella, N. C. Carpita, T. Carr, J.-G. Chen, T. R. Cooke et al.
Mol Syst Biol 7, 532-532 (23 July 2014)

Arabidopsis heterotrimeric G protein {beta} subunit, AGB1, regulates brassinosteroid signalling independently of BZR1
D. Tsugama, S. Liu, and T. Takano
J Exp Bot 64, 3213-3223 (1 August 2013)

Susceptibility of Intact Germinating Arabidopsis thaliana to Human Fungal Pathogens Cryptococcus neoformans and C. gattii
K. M. Warpeha, Y.-D. Park, and P. R. Williamson
Appl. Environ. Microbiol. 79, 2979-2988 (1 May 2013)

Heterotrimeric G protein signalling in the plant kingdom
D. Urano, J.-G. Chen, J. R. Botella, and A. M. Jones
Open Biol 3, 120186-120186 (27 March 2013)

Dissecting Arabidopsis G{beta} Signal Transduction on the Protein Surface
K. Jiang, A. Frick-Cheng, Y. Trusov, M. Delgado-Cerezo, D. M. Rosenthal, J. Lorek, R. Panstruga, F. L. Booker, J. R. Botella, A. Molina et al.
Plant Physiol. 159, 975-983 (1 July 2012)

The TvPirin Gene Is Necessary for Haustorium Development in the Parasitic Plant Triphysaria versicolor
P. C. G. Bandaranayake, A. Tomilov, N. B. Tomilova, Q. A. Ngo, N. Wickett, C. W. dePamphilis, and J. I. Yoder
Plant Physiol. 158, 1046-1053 (1 February 2012)

First off the mark: early seed germination
K. Weitbrecht, K. Muller, and G. Leubner-Metzger
J Exp Bot 62, 3289-3309 (1 June 2011)

Function and Expression Pattern of the {alpha} Subunit of the Heterotrimeric G Protein in Rice
Y. Izawa, Y. Takayanagi, N. Inaba, Y. Abe, M. Minami, Y. Fujisawa, H. Kato, S. Ohki, H. Kitano, Y. Iwasaki et al.
Plant Cell Physiol 51, 271-281 (1 February 2010)

Loss-of-Function Mutations in the Arabidopsis Heterotrimeric G-protein {alpha} Subunit Enhance the Developmental Defects of Brassinosteroid Signaling and Biosynthesis Mutants
Y. Gao, S. Wang, T. Asami, and J.-G. Chen
Plant Cell Physiol 49, 1013-1024 (1 July 2008)

G{gamma}1 + G{gamma}2 != G{beta}: Heterotrimeric G Protein G{gamma}-Deficient Mutants Do Not Recapitulate All Phenotypes of G{beta}-Deficient Mutants
Y. Trusov, W. Zhang, S. M. Assmann, and J. R. Botella
Plant Physiol. 147, 636-649 (1 June 2008)

Characterization of the Arabidopsis Heterotrimeric G Protein
S. Wang, S. M. Assmann, and N. V. Fedoroff
J Biol Chem 283, 13913-13922 (16 May 2008)

Abscisic Acid Receptors: Multiple Signal-perception Sites
X.-F. Wang, and D.-P. Zhang
Ann Bot 101, 311-317 (1 February 2008)

Heterotrimeric G Protein {gamma} Subunits Provide Functional Selectivity in G{beta}{gamma} Dimer Signaling in Arabidopsis
Y. Trusov, J. E. Rookes, K. Tilbrook, D. Chakravorty, M. G. Mason, D. Anderson, J.-G. Chen, A. M. Jones, and J. R. Botella
Plant Cell 19, 1235-1250 (1 April 2007)

The GCR1, GPA1, PRN1, NF-Y Signal Chain Mediates Both Blue Light and Abscisic Acid Responses in Arabidopsis
K. M. Warpeha, S. Upadhyay, J. Yeh, J. Adamiak, S. I. Hawkins, Y. R. Lapik, M. B. Anderson, and L. S. Kaufman
Plant Physiol. 143, 1590-1600 (1 April 2007)

Pirin Regulates Pyruvate Catabolism by Interacting with the Pyruvate Dehydrogenase E1 Subunit and Modulating Pyruvate Dehydrogenase Activity
P.-C. Soo, Y.-T. Horng, M.-J. Lai, J.-R. Wei, S.-C. Hsieh, Y.-L. Chang, Y.-H. Tsai, and H.-C. Lai
J. Bacteriol. 189, 109-118 (1 January 2007)

A Golgi-localized Hexose Transporter Is Involved in Heterotrimeric G Protein-mediated Early Development in Arabidopsis
H. X. Wang, R. R. Weerasinghe, T. D. Perdue, N. G. Cakmakci, J. P. Taylor, W. F. Marzluff, and A. M. Jones
Mol. Biol. Cell 17, 4257-4269 (1 October 2006)

Differential Roles of Arabidopsis Heterotrimeric G-Protein Subunits in Modulating Cell Division in Roots
J.-G. Chen, Y. Gao, and A. M. Jones
Plant Physiol. 141, 887-897 (1 July 2006)

Overexpression of the regulator of G-protein signalling protein enhances ABA-mediated inhibition of root elongation and drought tolerance in Arabidopsis
Y. Chen, F. Ji, H. Xie, and J. Liang
J Exp Bot 57, 2101-2110 (1 June 2006)

The Plastid Protein THYLAKOID FORMATION1 and the Plasma Membrane G-Protein GPA1 Interact in a Novel Sugar-Signaling Mechanism in Arabidopsis
J. Huang, J. P. Taylor, J.-G. Chen, J. F. Uhrig, D. J. Schnell, T. Nakagawa, K. L. Korth, and A. M. Jones
Plant Cell 18, 1226-1238 (1 May 2006)

G-Protein Complex Mutants Are Hypersensitive to Abscisic Acid Regulation of Germination and Postgermination Development
S. Pandey, J.-G. Chen, A. M. Jones, and S. M. Assmann
Plant Physiol. 141, 243-256 (1 May 2006)

G-Protein-Coupled Receptor 1, G-Protein G{alpha}-Subunit 1, and Prephenate Dehydratase 1 Are Required for Blue Light-Induced Production of Phenylalanine in Etiolated Arabidopsis
K. M. Warpeha, S. S. Lateef, Y. Lapik, M. Anderson, B.-S. Lee, and L. S. Kaufman
Plant Physiol. 140, 844-855 (1 March 2006)

Repressing the Expression of the SUCROSE NONFERMENTING-1-RELATED PROTEIN KINASE Gene in Pea Embryo Causes Pleiotropic Defects of Maturation Similar to an Abscisic Acid-Insensitive Phenotype
R. Radchuk, V. Radchuk, W. Weschke, L. Borisjuk, and H. Weber
Plant Physiol. 140, 263-278 (1 January 2006)

The Regulator of G-Protein Signaling Proteins Involved in Sugar and Abscisic Acid Signaling in Arabidopsis Seed Germination
Y. Chen, F. Ji, H. Xie, J. Liang, and J. Zhang
Plant Physiol. 140, 302-310 (1 January 2006)

G Protein Signaling in the Regulation of Arabidopsis Seed Germination
S. M. Assmann, F. Ji, H. Xie, J. Liang, and J. Zhang
Sci Signal 2005, cm11-cm11 (1 November 2005)

G Proteins Go Green: A Plant G Protein Signaling FAQ Sheet
S. M. Assmann, F. Ji, H. Xie, J. Liang, and J. Zhang
Science 310, 71-73 (7 October 2005)

The Mutant of sll1961, Which Encodes a Putative Transcriptional Regulator, Has a Defect in Regulation of Photosystem Stoichiometry in the Cyanobacterium Synechocystis sp. PCC 6803
T. Fujimori, M. Higuchi, H. Sato, H. Aiba, M. Muramatsu, Y. Hihara, and K. Sonoike
Plant Physiol. 139, 408-416 (1 September 2005)

Structural and Biochemical Analysis Reveal Pirins to Possess Quercetinase Activity
M. Adams, and Z. Jia
J Biol Chem 280, 28675-28682 (5 August 2005)

Different Signaling and Cell Death Roles of Heterotrimeric G Protein {alpha} and {beta} Subunits in the Arabidopsis Oxidative Stress Response to Ozone
J. H. Joo, S. Wang, J.G. Chen, A.M. Jones, and N. V. Fedoroff
Plant Cell 17, 957-970 (1 March 2005)

Study of the Constitutively Active Form of the {alpha} Subunit of Rice Heterotrimeric G Proteins
K. Oki, Y. Fujisawa, H. Kato, and Y. Iwasaki
Plant Cell Physiol 46, 381-386 (1 February 2005)

Plant G Proteins, Phytohormones, and Plasticity: Three Questions and a Speculation
S. M. Assmann, Y. Fujisawa, H. Kato, and Y. Iwasaki
Sci Signal 2004, re20-re20 (21 December 2004)

GCR1 Can Act Independently of Heterotrimeric G-Protein in Response to Brassinosteroids and Gibberellins in Arabidopsis Seed Germination
J.-G. Chen, S. Pandey, J. Huang, J. M. Alonso, J. R. Ecker, S. M. Assmann, and A. M. Jones
Plant Physiol. 135, 907-915 (1 June 2004)

The Arabidopsis Putative G Protein-Coupled Receptor GCR1 Interacts with the G Protein {alpha} Subunit GPA1 and Regulates Abscisic Acid Signaling
S. Pandey, and S. M. Assmann
Plant Cell 16, 1616-1632 (1 June 2004)

Plants: the latest model system for G-protein research
A. M. Jones, and S. M. Assmann
EMBO Rep. 5, 572-578 (1 June 2004)

Arabidopsis Phospholipase D{alpha}1 Interacts with the Heterotrimeric G-protein {alpha}-Subunit through a Motif Analogous to the DRY Motif in G-protein-coupled Receptors
J. Zhao, and X. Wang
J Biol Chem 279, 1794-1800 (16 January 2004)

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882