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Components of the Arabidopsis mRNA Decapping Complex Are Required for Early Seedling Development

Plant Cell, 1 May 2007
Vol. 19, Issue 5, p. 1549-1564
DOI: 10.1105/tpc.106.047621

Components of the Arabidopsis mRNA Decapping Complex Are Required for Early Seedling Development

  1. David C. Goeres1,
  2. Jaimie M. Van Norman1,
  3. Weiping Zhang,
  4. Nellie A. Fauver,
  5. Mary Lou Spencer and
  6. Leslie E. Sieburth2
  1. Department of Biology, University of Utah, Salt Lake City, Utah 84112
  1. 2To whom correspondence should be addressed. E-mail sieburth{at}; fax 801-581-4668.


To understand the mechanisms controlling vein patterning in Arabidopsis thaliana, we analyzed two phenotypically similar mutants, varicose (vcs) and trident (tdt). We had previously identified VCS, and recently, human VCS was shown to function in mRNA decapping. Here, we report that TDT encodes the mRNA-decapping enzyme. VCS and TDT function together in small cytoplasmic foci that appear to be processing bodies. To understand the developmental requirements for mRNA decapping, we characterized the vcs and tdt phenotypes. These mutants were small and chlorotic, with severe defects in shoot apical meristem formation and cotyledon vein patterning. Many capped mRNAs accumulated in tdt and vcs mutants, but surprisingly, some mRNAs were specifically depleted. In addition, loss of decapping arrested the decay of some mRNAs, while others showed either modest or no decay defects, suggesting that mRNAs may show specificity for particular decay pathways (3′ to 5′ and 5′ to 3′). Furthermore, the severe block to postembryonic development in vcs and tdt and the accompanying accumulation of embryonic mRNAs indicate that decapping is important for the embryo-to-seedling developmental transition.

  • Received October 10, 2006.
  • Revised April 17, 2007.
  • Accepted April 26, 2007.
  • Published May 18, 2007.


D. C. Goeres, J. M. Van Norman, W. Zhang, N. A. Fauver, M. L. Spencer, and L. E. Sieburth, Components of the Arabidopsis mRNA Decapping Complex Are Required for Early Seedling Development. Plant Cell 19, 1549-1564 (2007).

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R. Merret, V. K. Nagarajan, M.-C. Carpentier, S. Park, J.-J. Favory, J. Descombin, C. Picart, Y.-y. Charng, P. J. Green, J.-M. Deragon et al.
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In plants, decapping prevents RDR6-dependent production of small interfering RNAs from endogenous mRNAs
A. E. Martinez de Alba, A. B. Moreno, M. Gabriel, A. C. Mallory, A. Christ, R. Bounon, S. Balzergue, S. Aubourg, D. Gautheret, M. D. Crespi et al.
Nucleic Acids Res 43, 2902-2913 (11 March 2015)

The mRNA decay factor PAT1 functions in a pathway including MAP kinase 4 and immune receptor SUMM2
M. E. Roux, M. W. Rasmussen, K. Palma, S. Lolle, A. M. Regue, G. Bethke, J. Glazebrook, W. Zhang, L. Sieburth, M. R. Larsen et al.
EMBO J. 34, 593-608 (4 March 2015)

Tudor Staphylococcal Nuclease Links Formation of Stress Granules and Processing Bodies with mRNA Catabolism in Arabidopsis
E. Gutierrez-Beltran, P. N. Moschou, A. P. Smertenko, and P. V. Bozhkov
Plant Cell 27, 926-943 (1 March 2015)

Diffuse Decapping Enzyme DCP2 Accumulates in DCP1 Foci Under Heat Stress in Arabidopsis thaliana
K. Motomura, Q. T. N. Le, T. Hamada, N. Kutsuna, S. Mano, M. Nishimura, and Y. Watanabe
Plant Cell Physiol 56, 107-115 (1 January 2015)

Uridylation prevents 3' trimming of oligoadenylated mRNAs
F. M. Sement, E. Ferrier, H. Zuber, R. Merret, M. Alioua, J.-M. Deragon, C. Bousquet-Antonelli, H. Lange, and D. Gagliardi
Nucleic Acids Res 41, 7115-7127 (1 August 2013)

Arabidopsis thaliana LSM proteins function in mRNA splicing and degradation
A. Golisz, P. J. Sikorski, K. Kruszka, and J. Kufel
Nucleic Acids Res 41, 6232-6249 (1 July 2013)

Biogenesis, Turnover, and Mode of Action of Plant MicroRNAs
K. Rogers, and X. Chen
Plant Cell 25, 2383-2399 (1 July 2013)

Cytoplasmic and nuclear quality control and turnover of single-stranded RNA modulate post-transcriptional gene silencing in plants
A. B. Moreno, A. E. Martinez de Alba, F. Bardou, M. D. Crespi, H. Vaucheret, A. Maizel, and A. C. Mallory
Nucleic Acids Res 41, 4699-4708 (1 April 2013)

Human mitochondrial RNA decay mediated by PNPase-hSuv3 complex takes place in distinct foci
L. S. Borowski, A. Dziembowski, M. S. Hejnowicz, P. P. Stepien, and R. J. Szczesny
Nucleic Acids Res 41, 1223-1240 (1 January 2013)

Loss of Function of Arabidopsis C-Terminal Domain Phosphatase-Like1 Activates Iron Deficiency Responses at the Transcriptional Level
E. Aksoy, I. S. Jeong, and H. Koiwa
Plant Physiol. 161, 330-345 (1 January 2013)

LSM Proteins Provide Accurate Splicing and Decay of Selected Transcripts to Ensure Normal Arabidopsis Development
C. Perea-Resa, T. Hernandez-Verdeja, R. Lopez-Cobollo, M. d. M. Castellano, and J. Salinas
Plant Cell 24, 4930-4947 (1 December 2012)

Dehydration stress activates Arabidopsis MPK6 to signal DCP1 phosphorylation
J. Xu, and N.-H. Chua
EMBO J. 31, 1975-1984 (18 April 2012)

Proteomic Analysis of Embryonic Proteins Synthesized from Long-Lived mRNAs During Germination of Rice Seeds
N. Sano, H. Permana, R. Kumada, Y. Shinozaki, T. Tanabata, T. Yamada, T. Hirasawa, and M. Kanekatsu
Plant Cell Physiol 53, 687-698 (1 April 2012)

Mating Pheromone in Cryptococcus neoformans Is Regulated by a Transcriptional/Degradative "Futile" Cycle
Y.-D. Park, J. Panepinto, S. Shin, P. Larsen, S. Giles, and P. R. Williamson
J Biol Chem 285, 34746-34756 (5 November 2010)

Plant virus-mediated induction of miR168 is associated with repression of ARGONAUTE1 accumulation
E. Varallyay, A. Valoczi, A. Agyi, J. Burgyan, and Z. Havelda
EMBO J. 29, 3507-3519 (20 October 2010)

Conserved RNaseII domain protein functions in cytoplasmic mRNA decay and suppresses Arabidopsis decapping mutant phenotypes
W. Zhang, C. Murphy, and L. E. Sieburth
Proc. Natl. Acad. Sci. USA 107, 15981-15985 (7 September 2010)

Arabidopsis Deadenylases AtCAF1a and AtCAF1b Play Overlapping and Distinct Roles in Mediating Environmental Stress Responses
J. W. Walley, D. R. Kelley, G. Nestorova, D. L. Hirschberg, and K. Dehesh
Plant Physiol. 152, 866-875 (1 February 2010)

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M. C. Pomeranz, C. Hah, P.-C. Lin, S. G. Kang, J. J. Finer, P. J. Blackshear, and J.-C. Jang
Plant Physiol. 152, 151-165 (1 January 2010)

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J. Xu, and N.-H. Chua
Plant Cell 21, 3270-3279 (1 October 2009)

Transcriptome-Wide Analysis of Uncapped mRNAs in Arabidopsis Reveals Regulation of mRNA Degradation
Y. Jiao, J. L. Riechmann, and E. M. Meyerowitz
Plant Cell 20, 2571-2585 (1 October 2008)

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H. Zhang, S. D. Rider, J. T. Henderson, M. Fountain, K. Chuang, V. Kandachar, A. Simons, H. J. Edenberg, J. Romero-Severson, W. M. Muir et al.
J Biol Chem 283, 22637-22648 (15 August 2008)

Widespread Translational Inhibition by Plant miRNAs and siRNAs
P. Brodersen, L. Sakvarelidze-Achard, M. Bruun-Rasmussen, P. Dunoyer, Y. Y. Yamamoto, L. Sieburth, and O. Voinnet
Science 320, 1185-1190 (30 May 2008)

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Y. Li, M.-G. Song, and M. Kiledjian
Mol. Cell. Biol. 28, 939-948 (1 February 2008)

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S. Katiyar-Agarwal, S. Gao, A. Vivian-Smith, and H. Jin
Genes Dev. 21, 3123-3134 (1 December 2007)

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