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Components of the Arabidopsis mRNA Decapping Complex Are Required for Early Seedling Development

Plant Cell, 1 May 2007
Vol. 19, Issue 5, p. 1549-1564
DOI: 10.1105/tpc.106.047621

Components of the Arabidopsis mRNA Decapping Complex Are Required for Early Seedling Development

  1. David C. Goeres1,
  2. Jaimie M. Van Norman1,
  3. Weiping Zhang,
  4. Nellie A. Fauver,
  5. Mary Lou Spencer and
  6. Leslie E. Sieburth2
  1. Department of Biology, University of Utah, Salt Lake City, Utah 84112
  1. 2To whom correspondence should be addressed. E-mail sieburth{at}biology.utah.edu; fax 801-581-4668.

Abstract

To understand the mechanisms controlling vein patterning in Arabidopsis thaliana, we analyzed two phenotypically similar mutants, varicose (vcs) and trident (tdt). We had previously identified VCS, and recently, human VCS was shown to function in mRNA decapping. Here, we report that TDT encodes the mRNA-decapping enzyme. VCS and TDT function together in small cytoplasmic foci that appear to be processing bodies. To understand the developmental requirements for mRNA decapping, we characterized the vcs and tdt phenotypes. These mutants were small and chlorotic, with severe defects in shoot apical meristem formation and cotyledon vein patterning. Many capped mRNAs accumulated in tdt and vcs mutants, but surprisingly, some mRNAs were specifically depleted. In addition, loss of decapping arrested the decay of some mRNAs, while others showed either modest or no decay defects, suggesting that mRNAs may show specificity for particular decay pathways (3′ to 5′ and 5′ to 3′). Furthermore, the severe block to postembryonic development in vcs and tdt and the accompanying accumulation of embryonic mRNAs indicate that decapping is important for the embryo-to-seedling developmental transition.

  • Received October 10, 2006.
  • Revised April 17, 2007.
  • Accepted April 26, 2007.
  • Published May 18, 2007.

Citation:

D. C. Goeres, J. M. Van Norman, W. Zhang, N. A. Fauver, M. L. Spencer, and L. E. Sieburth, Components of the Arabidopsis mRNA Decapping Complex Are Required for Early Seedling Development. Plant Cell 19, 1549-1564 (2007).

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