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Eosinophil granules function extracellularly as receptor-mediated secretory organelles

PNAS, 25 November 2008
Vol. 105, Issue 47, p. 18478-18483
DOI: 10.1073/pnas.0804547105

Eosinophil granules function extracellularly as receptor-mediated secretory organelles

  1. Josiane S. Nevesa,1,
  2. Sandra A. C. Pereza,b,1,
  3. Lisa A. Spencera,
  4. Rossana C. N. Meloa,c,d,
  5. Lauren Reynoldsa,
  6. Ionita Ghirana,
  7. Salahaddin Mahmudi-Azere,
  8. Solomon O. Odemuyiwae,
  9. Ann M. Dvorakd,
  10. Redwan Moqbele,2, and
  11. Peter F. Wellera,2,3
  1. Departments of aMedicine and
  2. dPathology, Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, MA, 02215;
  3. bLaboratory of Inflammation, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, RJ, 21045-900, Brazil;
  4. cDepartment of Biology, Federal University of Juiz de Fora Juiz de Fora, MG, 36036-330 Brazil; and
  5. ePulmonary Research Group, Department of Medicine, University of Alberta, Edmonton, Alberta, Canada
  1. Edited by K. Frank Austen, Brigham and Women's Hospital, Boston, MA, and approved September 29, 2008

  2. 1J.S.N. and S.A.C.P. contributed equally to this work.

  3. 2R.M. and P.F.W. contributed equally to this work. (received for review May 12, 2008)

Abstract

Intracellular granules in several types of leukocytes contain preformed proteins whose secretions contribute to immune and inflammatory functions of leukocytes, including eosinophils, cells notably associated with asthma, allergic inflammation, and helminthic infections. Cytokines and chemokines typically elicit extracellular secretion of granule proteins by engaging receptors expressed externally on the plasma membranes of cells, including eosinophils. Eosinophil granules, in addition to being intracellular organelles, are found as intact membrane-bound structures extracellularly in tissue sites of eosinophil-associated diseases. Neither the secretory capacities of cell-free eosinophil granules nor the presence of functional cytokine and chemokine receptors on membranes of leukocyte granules have been recognized. Here, we show that granules of human eosinophils express membrane receptors for a cytokine, IFN-γ, and G protein–coupled membrane receptors for a chemokine, eotaxin, and that these receptors function by activating signal-transducing pathways within granules to elicit secretion from within granules. Capacities of intracellular granule organelles to function autonomously outside of eosinophils as independent, ligand-responsive, secretion-competent structures constitute a novel postcytolytic mechanism for regulated secretion of eosinophil granule proteins that may contribute to eosinophil-mediated inflammation and immunomodulation.

  • allergy
  • specific
  • eotaxin
  • IFN-γ

Footnotes

  • 3To whom correspondence should be addressed. E-mail: pweller{at}bidmc.harvard.edu
  • Author contributions: J.S.N., S.A.C.P., S.M.-A., S.O.O., A.M.D., R.M., and P.F.W. designed research; J.S.N., S.A.C.P., L.A.S., R.C.N.M., L.R., and I.G. performed research; J.S.N., S.A.C.P., R.M., and P.F.W. analyzed data; and J.S.N., R.M., and P.F.W. wrote the paper.

  • The authors declare no conflict of interest.

  • This article is a PNAS Direct Submission.

  • This article contains supporting information online at www.pnas.org/cgi/content/full/0804547105/DCSupplemental.

Citation:

J. S. Neves, S. A. Perez, L. A. Spencer, R. C. Melo, L. Reynolds, I. Ghiran, S. Mahmudi-Azer, S. O. Odemuyiwa, A. M. Dvorak, R. Moqbel, and P. F. Weller, Eosinophil granules function extracellularly as receptor-mediated secretory organelles. PNAS 105, 18478-18483 (2008).

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