Error message

No crossref credentials set for sci

Role of Hec1 in Spindle Checkpoint Signaling and Kinetochore Recruitment of Mad1/Mad2

Science, 27 September 2002
Vol. 297, Issue 5590, p. 2267-2270
DOI: 10.1126/science.1075596

Role of Hec1 in Spindle Checkpoint Signaling and Kinetochore Recruitment of Mad1/Mad2

  1. Silvia Martin-Lluesma,
  2. Volker M. Stucke,
  3. Erich A. Nigg*
  1. Department of Cell Biology, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18a, D-82152 Martinsried, Germany.


The spindle checkpoint delays sister chromatid separation until all chromosomes have undergone bipolar spindle attachment. Checkpoint failure may result in chromosome mis-segregation and may contribute to tumorigenesis. We showed that the human protein Hec1 was required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores. Depletion of Hec1 impaired chromosome congression and caused persistent activation of the spindle checkpoint, indicating that high steady-state levels of Mad1/Mad2 complexes at kinetochores were not essential for checkpoint signaling. Simultaneous depletion of Hec1 and Mad2 caused catastrophic mitotic exit, making Hec1 an attractive target for the selective elimination of spindle checkpoint–deficient cells.

  • * To whom correspondence should be addressed. E-mail: nigg{at}

  • Received for publication 1 July 2001.
  • Accepted for publication 13 August 2002.


S. Martin-Lluesma, V. M. Stucke, and E. A. Nigg, Role of Hec1 in Spindle Checkpoint Signaling and Kinetochore Recruitment of Mad1/Mad2. Science 297, 2267-2270 (2002).

Single-protein spin resonance spectroscopy under ambient conditions
F. Shi, Q. Zhang, P. Wang, H. Sun, J. Wang, X. Rong, M. Chen, C. Ju, F. Reinhard, H. Chen et al.
Science 347, 1135-1138 (6 March 2015)

Multiple assembly mechanisms anchor the KMN spindle checkpoint platform at human mitotic kinetochores
S. Kim, and H. Yu
JCB 208, 181-196 (19 January 2015)

Mad2, Bub3, and Mps1 regulate chromosome segregation and mitotic synchrony in Giardia intestinalis, a binucleate protist lacking an anaphase-promoting complex
J.-J. Vicente, and W. Z. Cande
Mol. Biol. Cell 25, 2774-2787 (15 September 2014)

Pathway redundancy and protein essentiality revealed in the Saccharomyces cerevisiae interaction networks
I. Ulitsky, and R. Shamir
Mol Syst Biol 3, 104-104 (28 July 2014)

The Kinetochore
I. M. Cheeseman, and R. Shamir
Cold Spring Harb. Perspect. Biol. 6, a015826-a015826 (1 July 2014)

Activity of a Novel Hec1-Targeted Anticancer Compound against Breast Cancer Cell Lines In Vitro and In Vivo
L. Y. L. Huang, C.-C. Chang, Y.-S. Lee, J.-M. Chang, J.-J. Huang, S.-H. Chuang, K.-J. Kao, G. M. G. Lau, P.-Y. Tsai, C.-W. Liu et al.
Molecular Cancer Therapeutics 13, 1419-1430 (1 June 2014)

Phosphorylation at Threonine 288 by Cell Cycle Checkpoint Kinase 2 (CHK2) Controls Human Monopolar Spindle 1 (Mps1) Kinetochore Localization
C.-W. Yeh, Z.-C. Yu, P.-H. Chen, Y.-C. Cheng, and S.-Y. Shieh
J Biol Chem 289, 15319-15327 (30 May 2014)

CENP-I and Aurora B act as a molecular switch that ties RZZ/Mad1 recruitment to kinetochore attachment status
D. R. Matson, and P. T. Stukenberg
JCB 205, 541-554 (26 May 2014)

A Bub1-Mad1 interaction targets the Mad1-Mad2 complex to unattached kinetochores to initiate the spindle checkpoint
M. W. Moyle, T. Kim, N. Hattersley, J. Espeut, D. K. Cheerambathur, K. Oegema, and A. Desai
JCB 204, 647-657 (3 March 2014)

Mad1 contribution to spindle assembly checkpoint signalling goes beyond presenting Mad2 at kinetochores
S. Heinrich, K. Sewart, H. Windecker, M. Langegger, N. Schmidt, N. Hustedt, and S. Hauf
EMBO Rep. 15, 291-298 (1 March 2014)

Kinetochore composition and its function: lessons from yeasts
Y. Yamagishi, T. Sakuno, Y. Goto, and Y. Watanabe
FEMS Microbiol Rev 38, 185-200 (1 March 2014)

Mad1 kinetochore recruitment by Mps1-mediated phosphorylation of Bub1 signals the spindle checkpoint
N. London, and S. Biggins
Genes Dev. 28, 140-152 (15 January 2014)

Phosphorylation of Microtubule-binding Protein Hec1 by Mitotic Kinase Aurora B Specifies Spindle Checkpoint Kinase Mps1 Signaling at the Kinetochore
T. Zhu, Z. Dou, B. Qin, C. Jin, X. Wang, L. Xu, Z. Wang, L. Zhu, F. Liu, X. Gao et al.
J Biol Chem 288, 36149-36159 (13 December 2013)

Monopolar Spindle 1 (MPS1) Kinase Promotes Production of Closed MAD2 (C-MAD2) Conformer and Assembly of the Mitotic Checkpoint Complex
A. R. Tipton, W. Ji, B. Sturt-Gillespie, M. E. Bekier, K. Wang, W. R. Taylor, and S.-T. Liu
J Biol Chem 288, 35149-35158 (6 December 2013)

The spindle assembly checkpoint: progress and persistent puzzles
S. Hauf, W. Ji, B. Sturt-Gillespie, M. E. Bekier, K. Wang, W. R. Taylor, and S.-T. Liu
Biochm. Soc. Trans. 41, 1755-1760 (1 December 2013)

Complementary Interhelical Interactions between Three Buried Glu-Lys Pairs within Three Heptad Repeats Are Essential for Hec1-Nuf2 Heterodimerization and Mitotic Progression
B. Ngo, C.-M. Hu, X. E. Guo, B. Ngo, R. Wei, J. Zhu, and W.-H. Lee
J Biol Chem 288, 34403-34413 (29 November 2013)

A TPR domain-containing N-terminal module of MPS1 is required for its kinetochore localization by Aurora B
W. Nijenhuis, E. von Castelmur, D. Littler, V. De Marco, E. Tromer, M. Vleugel, M. H. J. van Osch, B. Snel, A. Perrakis, G. J. P. L. Kops et al.
JCB 201, 217-231 (15 April 2013)

CENP-T provides a structural platform for outer kinetochore assembly
T. Nishino, F. Rago, T. Hori, K. Tomii, I. M. Cheeseman, and T. Fukagawa
EMBO J. 32, 424-436 (6 February 2013)

Structural and functional insights into the role of the N-terminal Mps1 TPR domain in the SAC (spindle assembly checkpoint)
P. Thebault, D. Y. Chirgadze, Z. Dou, T. L. Blundell, S. Elowe, and V. M. Bolanos-Garcia
Biochem. J. 448, 321-328 (15 December 2012)

The KMN protein network - chief conductors of the kinetochore orchestra
D. Varma, and E. D. Salmon
J. Cell Sci. 125, 5927-5936 (15 December 2012)

Mph1 kinetochore localization is crucial and upstream in the hierarchy of spindle assembly checkpoint protein recruitment to kinetochores
S. Heinrich, H. Windecker, N. Hustedt, and S. Hauf
J. Cell Sci. 125, 4720-4727 (15 October 2012)

Human chromokinesins promote chromosome congression and spindle microtubule dynamics during mitosis
C. Wandke, M. Barisic, R. Sigl, V. Rauch, F. Wolf, A. C. Amaro, C. H. Tan, A. J. Pereira, U. Kutay, H. Maiato et al.
JCB 198, 847-863 (3 September 2012)

CENP-E-dependent BubR1 autophosphorylation enhances chromosome alignment and the mitotic checkpoint
Y. Guo, C. Kim, S. Ahmad, J. Zhang, and Y. Mao
JCB 198, 205-217 (23 July 2012)

The Ndc80 internal loop is required for recruitment of the Ska complex to establish end-on microtubule attachment to kinetochores
G. Zhang, C. D. Kelstrup, X.-W. Hu, M. J. Kaas Hansen, M. R. Singleton, J. V. Olsen, and J. Nilsson
J. Cell Sci. 125, 3243-3253 (1 July 2012)

Spindle checkpoint-independent inhibition of mitotic chromosome segregation by Drosophila Mps1
F. Althoff, R. E. Karess, and C. F. Lehner
Mol. Biol. Cell 23, 2275-2291 (15 June 2012)

Structure of human Mad1 C-terminal domain reveals its involvement in kinetochore targeting
S. Kim, H. Sun, D. R. Tomchick, H. Yu, and X. Luo
Proc. Natl. Acad. Sci. USA 109, 6549-6554 (24 April 2012)

RED, a Spindle Pole-associated Protein, Is Required for Kinetochore Localization of MAD1, Mitotic Progression, and Activation of the Spindle Assembly Checkpoint
P.-C. Yeh, C.-C. Yeh, Y.-C. Chen, and Y.-L. Juang
J Biol Chem 287, 11704-11716 (6 April 2012)

Characterization of Spindle Checkpoint Kinase Mps1 Reveals Domain with Functional and Structural Similarities to Tetratricopeptide Repeat Motifs of Bub1 and BubR1 Checkpoint Kinases
S. Lee, P. Thebault, L. Freschi, S. Beaufils, T. L. Blundell, C. R. Landry, V. M. Bolanos-Garcia, and S. Elowe
J Biol Chem 287, 5988-6001 (17 February 2012)

CENP-E Kinesin Interacts with SKAP Protein to Orchestrate Accurate Chromosome Segregation in Mitosis
Y. Huang, W. Wang, P. Yao, X. Wang, X. Liu, X. Zhuang, F. Yan, J. Zhou, J. Du, T. Ward et al.
J Biol Chem 287, 1500-1509 (6 January 2012)

Centromere-tethered Mps1 pombe homolog (Mph1) kinase is a sufficient marker for recruitment of the spindle checkpoint protein Bub1, but not Mad1
D. Ito, Y. Saito, and T. Matsumoto
Proc. Natl. Acad. Sci. USA 109, 209-214 (3 January 2012)

Spindle assembly checkpoint: the third decade
A. Musacchio, Y. Saito, and T. Matsumoto
Phil Trans R Soc B 366, 3595-3604 (27 December 2011)

p31comet acts to ensure timely spindle checkpoint silencing subsequent to kinetochore attachment
R. S. Hagan, M. S. Manak, H. K. Buch, M. G. Meier, P. Meraldi, J. V. Shah, P. K. Sorger, and S. J. Doxsey
Mol. Biol. Cell 22, 4236-4246 (15 November 2011)

Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint
R. Wei, B. Ngo, G. Wu, and W.-H. Lee
Mol. Biol. Cell 22, 3584-3594 (1 October 2011)

Probing the in vivo function of Mad1:C-Mad2 in the spindle assembly checkpoint
L. L. Fava, M. Kaulich, E. A. Nigg, and A. Santamaria
EMBO J. 30, 3322-3336 (17 August 2011)

Shugoshin is a Mad1/Cdc20-like interactor of Mad2
M. Orth, B. Mayer, K. Rehm, U. Rothweiler, D. Heidmann, T. A. Holak, and O. Stemmann
EMBO J. 30, 2868-2880 (20 July 2011)

Physiological Relevance of Cell Cycle Kinases
M. Malumbres, B. Mayer, K. Rehm, U. Rothweiler, D. Heidmann, T. A. Holak, and O. Stemmann
Physiol. Rev. 91, 973-1007 (1 July 2011)

A mitotic role for Mad1 beyond the spindle checkpoint
D. Emre, R. Terracol, A. Poncet, Z. Rahmani, and R. E. Karess
J. Cell Sci. 124, 1664-1671 (15 May 2011)

Evidence that Aurora B is implicated in spindle checkpoint signalling independently of error correction
S. Santaguida, C. Vernieri, F. Villa, A. Ciliberto, and A. Musacchio
EMBO J. 30, 1508-1519 (20 April 2011)

The Ndc80 complex uses a tripartite attachment point to couple microtubule depolymerization to chromosome movement
J. G. Tooley, S. A. Miller, and P. T. Stukenberg
Mol. Biol. Cell 22, 1217-1226 (15 April 2011)

The NDC80 complex proteins Nuf2 and Hec1 make distinct contributions to kinetochore-microtubule attachment in mitosis
L. J. R. Sundin, G. J. Guimaraes, and J. G. DeLuca
Mol. Biol. Cell 22, 759-768 (15 March 2011)

Temporal changes in Hec1 phosphorylation control kinetochore-microtubule attachment stability during mitosis
K. F. DeLuca, S. M. A. Lens, and J. G. DeLuca
J. Cell Sci. 124, 622-634 (15 February 2011)

CENP-U Cooperates with Hec1 to Orchestrate Kinetochore-Microtubule Attachment
S. Hua, Z. Wang, K. Jiang, Y. Huang, T. Ward, L. Zhao, Z. Dou, and X. Yao
J Biol Chem 286, 1627-1638 (14 January 2011)

Drosophila Mis12 Complex Acts as a Single Functional Unit Essential for Anaphase Chromosome Movement and a Robust Spindle Assembly Checkpoint
Z. Venkei, M. R. Przewloka, and D. M. Glover
Genetics 187, 131-140 (1 January 2011)

Driving chromosome segregation: lessons from the human and Drosophila centromere-kinetochore machinery
B. Orr, O. Afonso, T. Feijao, and C. E. Sunkel
Biochm. Soc. Trans. 38, 1667-1675 (1 December 2010)

CLASP1, astrin and Kif2b form a molecular switch that regulates kinetochore-microtubule dynamics to promote mitotic progression and fidelity
A. L. Manning, S. F. Bakhoum, S. Maffini, C. Correia-Melo, H. Maiato, and D. A. Compton
EMBO J. 29, 3531-3543 (20 October 2010)

Release of Mps1 from kinetochores is crucial for timely anaphase onset
N. Jelluma, T. B. Dansen, T. Sliedrecht, N. P. Kwiatkowski, and G. J. P. L. Kops
JCB 191, 281-290 (18 October 2010)

Aurora B kinase controls the targeting of the Astrin-SKAP complex to bioriented kinetochores
J. C. Schmidt, T. Kiyomitsu, T. Hori, C. B. Backer, T. Fukagawa, and I. M. Cheeseman
JCB 191, 269-280 (18 October 2010)

SPICE - a previously uncharacterized protein required for centriole duplication and mitotic chromosome congression
M. Archinti, C. Lacasa, N. Teixido-Travesa, and J. Luders
J. Cell Sci. 123, 3039-3046 (15 September 2010)

Sustained Mps1 activity is required in mitosis to recruit O-Mad2 to the Mad1-C-Mad2 core complex
L. Hewitt, A. Tighe, S. Santaguida, A. M. White, C. D. Jones, A. Musacchio, S. Green, and S. S. Taylor
JCB 190, 25-34 (12 July 2010)

Spindly/CCDC99 Is Required for Efficient Chromosome Congression and Mitotic Checkpoint Regulation
M. Barisic, B. Sohm, P. Mikolcevic, C. Wandke, V. Rauch, T. Ringer, M. Hess, G. Bonn, and S. Geley
Mol. Biol. Cell 21, 1968-1981 (15 June 2010)

Welcome to a new kind of tension: translating kinetochore mechanics into a wait-anaphase signal
T. J. Maresca, and E. D. Salmon
J. Cell Sci. 123, 825-835 (15 March 2010)

Hec1 Contributes to Mitotic Centrosomal Microtubule Growth for Proper Spindle Assembly through Interaction with Hice1
G. Wu, R. Wei, E. Cheng, B. Ngo, and W.-H. Lee
Mol. Biol. Cell 20, 4686-4695 (15 November 2009)

Elevating the frequency of chromosome mis-segregation as a strategy to kill tumor cells
A. Janssen, G. J. P. L. Kops, and R. H. Medema
Proc. Natl. Acad. Sci. USA 106, 19108-19113 (10 November 2009)

Enhanced genomic instabilities caused by deregulated microtubule dynamics and chromosome segregation: a perspective from genetic studies in mice
C. V. Rao, H. Y. Yamada, Y. Yao, and W. Dai
Carcinogenesis 30, 1469-1474 (1 September 2009)

A quantitative systems view of the spindle assembly checkpoint
A. Ciliberto, and J. V. Shah
EMBO J. 28, 2162-2173 (5 August 2009)

Kinase Signaling in the Spindle Checkpoint
J. Kang, and H. Yu
J Biol Chem 284, 15359-15363 (5 June 2009)

Spindle assembly checkpoint-related failure perturbs early embryonic divisions and reduces reproductive performance of LT/Sv mice
Z. Maciejewska, Z. Polanski, K. Kisiel, J. Z Kubiak, and M. A Ciemerych
Reproduction 137, 931-942 (1 June 2009)

Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly
Y. W. Chan, L. L. Fava, A. Uldschmid, M. H.A. Schmitz, D. W. Gerlich, E. A. Nigg, and A. Santamaria
JCB 185, 859-874 (1 June 2009)

Stable kinetochore-microtubule interactions depend on the Ska complex and its new component Ska3/C13Orf3
T. N. Gaitanos, A. Santamaria, A. A. Jeyaprakash, B. Wang, E. Conti, and E. A. Nigg
EMBO J. 28, 1442-1452 (20 May 2009)

Mimicking Ndc80 phosphorylation triggers spindle assembly checkpoint signalling
S. Kemmler, M. Stach, M. Knapp, J. Ortiz, J. Pfannstiel, T. Ruppert, and J. Lechner
EMBO J. 28, 1099-1110 (22 April 2009)

Dynein light intermediate chain 1 is required for progress through the spindle assembly checkpoint
M. V. S. Sivaram, T. L. Wadzinski, S. D. Redick, T. Manna, and S. J. Doxsey
EMBO J. 28, 902-914 (8 April 2009)

The nuclear export factor Xpo1p targets Mad1p to kinetochores in yeast
R. J. Scott, L. V. Cairo, D. W. Van de Vosse, and R. W. Wozniak
JCB 184, 21-29 (12 January 2009)

Regulation of Kinetochore Recruitment of Two Essential Mitotic Spindle Checkpoint Proteins by Mps1 Phosphorylation
Q. Xu, S. Zhu, W. Wang, X. Zhang, W. Old, N. Ahn, and X. Liu
Mol. Biol. Cell 20, 10-20 (1 January 2009)

Dynein, Lis1 and CLIP-170 counteract Eg5-dependent centrosome separation during bipolar spindle assembly
M. E. Tanenbaum, L. Macurek, N. Galjart, and R. H. Medema
EMBO J. 27, 3235-3245 (17 December 2008)

MCAK-Independent Functions of ch-Tog/XMAP215 in Microtubule Plus-End Dynamics
A. R. Barr, and F. Gergely
Mol. Cell. Biol. 28, 7199-7211 (1 December 2008)

Hec1 overexpression hyperactivates the mitotic checkpoint and induces tumor formation in vivo
E. Diaz-Rodriguez, R. Sotillo, J.-M. Schvartzman, and R. Benezra
Proc. Natl. Acad. Sci. USA 105, 16719-16724 (28 October 2008)

Mps1 kinase activity restrains anaphase during an unperturbed mitosis and targets Mad2 to kinetochores
A. Tighe, O. Staples, and S. Taylor
JCB 181, 893-901 (21 October 2008)

Small Molecule Targeting the Hec1/Nek2 Mitotic Pathway Suppresses Tumor Cell Growth in Culture and in Animal
G. Wu, X.-L. Qiu, L. Zhou, J. Zhu, R. Chamberlin, J. Lau, P.-L. Chen, and W.-H. Lee
Cancer Res. 68, 8393-8399 (15 October 2008)

Dual Regulation of Mad2 Localization on Kinetochores by Bub1 and Dam1/DASH that Ensure Proper Spindle Interaction
S. Saitoh, Y. Kobayashi, Y. Ogiyama, and K. Takahashi
Mol. Biol. Cell 19, 3885-3897 (1 September 2008)

Spindly attachments
F. Civril, and A. Musacchio
Genes Dev. 22, 2302-2307 (1 September 2008)

Distinct Sequence Elements of Cyclin B1 Promote Localization to Chromatin, Centrosomes, and Kinetochores during Mitosis
A. M. Bentley, G. Normand, J. Hoyt, and R. W. King
Mol. Biol. Cell 18, 4847-4858 (1 December 2007)

The Transforming Acidic Coiled Coil 3 Protein Is Essential for Spindle-dependent Chromosome Alignment and Mitotic Survival
L. Schneider, F. Essmann, A. Kletke, P. Rio, H. Hanenberg, W. Wetzel, K. Schulze-Osthoff, B. Nurnberg, and R. P. Piekorz
J Biol Chem 282, 29273-29283 (5 October 2007)

Tension-sensitive Plk1 phosphorylation on BubR1 regulates the stability of kinetochore microtubule interactions
S. Elowe, S. Hummer, A. Uldschmid, X. Li, and E. A. Nigg
Genes Dev. 21, 2205-2219 (1 September 2007)

BubR1 and APC/EB1 cooperate to maintain metaphase chromosome alignment
J. Zhang, S. Ahmad, and Y. Mao
JCB 178, 773-784 (27 August 2007)

Human NUF2 Interacts with Centromere-associated Protein E and Is Essential for a Stable Spindle Microtubule-Kinetochore Attachment
D. Liu, X. Ding, J. Du, X. Cai, Y. Huang, T. Ward, A. Shaw, Y. Yang, R. Hu, C. Jin et al.
J Biol Chem 282, 21415-21424 (20 July 2007)

BUB1 mediation of caspase-independent mitotic death determines cell fate
Y. Niikura, A. Dixit, R. Scott, G. Perkins, and K. Kitagawa
JCB 178, 283-296 (10 July 2007)

Polo-like Kinase 1 Facilitates Chromosome Alignment during Prometaphase through BubR1
S. Matsumura, F. Toyoshima, and E. Nishida
J Biol Chem 282, 15217-15227 (18 May 2007)

The human Nup107-160 nuclear pore subcomplex contributes to proper kinetochore functions
M. Zuccolo, A. Alves, V. Galy, S. Bolhy, E. Formstecher, V. Racine, J.-B. Sibarita, T. Fukagawa, R. Shiekhattar, T. Yen et al.
EMBO J. 26, 1853-1864 (4 April 2007)

Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska2
A. Hanisch, H. H. Sillje, and E. A. Nigg
EMBO J. 25, 5504-5515 (29 November 2006)

Activation of CDCA1-KNTC2, Members of Centromere Protein Complex, Involved in Pulmonary Carcinogenesis
S. Hayama, Y. Daigo, T. Kato, N. Ishikawa, T. Yamabuki, M. Miyamoto, T. Ito, E. Tsuchiya, S. Kondo, Y. Nakamura et al.
Cancer Res. 66, 10339-10348 (1 November 2006)

Mapping the assembly pathways that specify formation of the trilaminar kinetochore plates in human cells
S.-T. Liu, J. B. Rattner, S. A. Jablonski, and T. J. Yen
JCB 175, 41-53 (9 October 2006)

Loading of the 3F3/2 Antigen onto Kinetochores Is Dependent on the Ordered Assembly of the Spindle Checkpoint Proteins
O. K. Wong, and G. Fang
Mol. Biol. Cell 17, 4390-4399 (1 October 2006)

Induction of apoptosis by monastrol, an inhibitor of the mitotic kinesin Eg5, is independent of the spindle checkpoint.
G. M. Chin, and R. Herbst
Molecular Cancer Therapeutics 5, 2580-2591 (1 October 2006)

The human kinetochore proteins Nnf1R and Mcm21R are required for accurate chromosome segregation
A. D. McAinsh, P. Meraldi, V. M. Draviam, A. Toso, and P. K. Sorger
EMBO J. 25, 4033-4049 (6 September 2006)

Phosphorylation- and Polo-Box-dependent Binding of Plk1 to Bub1 Is Required for the Kinetochore Localization of Plk1
W. Qi, Z. Tang, and H. Yu
Mol. Biol. Cell 17, 3705-3716 (1 August 2006)

Bmi-1 Is a Novel Molecular Marker of Nasopharyngeal Carcinoma Progression and Immortalizes Primary Human Nasopharyngeal Epithelial Cells.
L.-B. Song, M.-S. Zeng, W.-T. Liao, L. Zhang, H.-Y. Mo, W.-L. Liu, J.-Y. Shao, Q.-L. Wu, M.-Z. Li, Y.-F. Xia et al.
Cancer Res. 66, 6225-6232 (15 June 2006)

Coordinated Requirements of Human Topo II and Cohesin for Metaphase Centromere Alignment under Mad2-dependent Spindle Checkpoint Surveillance
Y. Toyoda, and M. Yanagida
Mol. Biol. Cell 17, 2287-2302 (1 May 2006)

DNA Damage-induced Expression of p53 Suppresses Mitotic Checkpoint Kinase hMps1: THE LACK OF THIS SUPPRESSION IN p53MUT CELLS CONTRIBUTES TO APOPTOSIS
M. R. Bhonde, M.-L. Hanski, J. Budczies, M. Cao, B. Gillissen, D. Moorthy, F. Simonetta, H. Scherubl, M. Truss, C. Hagemeier et al.
J Biol Chem 281, 8675-8685 (31 March 2006)

Different Plk1 Functions Show Distinct Dependencies on Polo-Box Domain-mediated Targeting
A. Hanisch, A. Wehner, E. A. Nigg, and H. H.W. Sillje
Mol. Biol. Cell 17, 448-459 (1 January 2006)

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882