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Role of Hec1 in Spindle Checkpoint Signaling and Kinetochore Recruitment of Mad1/Mad2

Science, 27 September 2002
Vol. 297, Issue 5590, p. 2267-2270
DOI: 10.1126/science.1075596

Role of Hec1 in Spindle Checkpoint Signaling and Kinetochore Recruitment of Mad1/Mad2

  1. Silvia Martin-Lluesma,
  2. Volker M. Stucke,
  3. Erich A. Nigg*
  1. Department of Cell Biology, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18a, D-82152 Martinsried, Germany.

Abstract

The spindle checkpoint delays sister chromatid separation until all chromosomes have undergone bipolar spindle attachment. Checkpoint failure may result in chromosome mis-segregation and may contribute to tumorigenesis. We showed that the human protein Hec1 was required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores. Depletion of Hec1 impaired chromosome congression and caused persistent activation of the spindle checkpoint, indicating that high steady-state levels of Mad1/Mad2 complexes at kinetochores were not essential for checkpoint signaling. Simultaneous depletion of Hec1 and Mad2 caused catastrophic mitotic exit, making Hec1 an attractive target for the selective elimination of spindle checkpoint–deficient cells.

  • * To whom correspondence should be addressed. E-mail: nigg{at}biochem.mpg.de

  • Received for publication 1 July 2001.
  • Accepted for publication 13 August 2002.

Citation:

S. Martin-Lluesma, V. M. Stucke, and E. A. Nigg, Role of Hec1 in Spindle Checkpoint Signaling and Kinetochore Recruitment of Mad1/Mad2. Science 297, 2267-2270 (2002).

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