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The Signal to Move: D. discoideum Go Orienteering

Science, 6 June 2003
Vol. 300, Issue 5625, p. 1525-1527
DOI: 10.1126/science.1085439

The Signal to Move: D. discoideum Go Orienteering

  1. Alan R. Kimmel1,
  2. Carole A. Parent2,*
  1. 1 Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892, USA.
  2. 2 Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
  1. * To whom correspondence should be addressed. E-mail: parentc{at}


Cells migrating directionally toward a chemoattractant source display a highly polarized cytoskeletal organization, with F-actin localized predominantly at the anterior and myosin II at the lateral and posterior regions. Dictyostelium discoideum has proven a useful system for elucidating signaling pathways that regulate this chemotactic response. During development, extracellular adenosine 3', 5' monophosphate (cAMP) functions as a primary signal to activate cell surface cAMP receptors (cARs). These receptors transduce different signals depending on whether or not they are coupled to heterotrimeric guanine nucleotide–binding proteins (G proteins) (see the STKE Connections Maps). Multiple G protein–stimulated pathways interact to establish polarity in chemotaxing D. discoideum cells by localizing F-actin at their leading edge and by regulating the phosphorylation state and assembly of myosin II. Many of the molecular interactions described are fundamental to the regulation of chemotaxis in other eukaryotic cells.


A. R. Kimmel and C. A. Parent, The Signal to Move: D. discoideum Go Orienteering. Science 300, 1525-1527 (2003).

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The interplay of cell-cell and cell-substrate adhesion in collective cell migration
C. Wang, S. Chowdhury, M. Driscoll, C. A. Parent, S. K. Gupta, and W. Losert
J R Soc Interface 11, 20140684-20140684 (6 November 2014)

Actin cross-linking proteins cortexillin I and II are required for cAMP signaling during Dictyostelium chemotaxis and development
S. Shu, X. Liu, P. W. Kriebel, M. P. Daniels, and E. D. Korn
Mol. Biol. Cell 23, 390-400 (15 January 2012)

Combinatorial cell-specific regulation of GSK3 directs cell differentiation and polarity in Dictyostelium
L. Kim, J. Brzostowski, A. Majithia, N.-S. Lee, V. McMains, and A. R. Kimmel
Development 138, 421-430 (1 February 2011)

Mobility of G proteins is heterogeneous and polarized during chemotaxis
F. van Hemert, M. D. Lazova, B. E. Snaar-Jagaska, and T. Schmidt
J. Cell Sci. 123, 2922-2930 (1 September 2010)

Cell speed, persistence and information transmission during signal relay and collective migration
C. P. McCann, P. W. Kriebel, C. A. Parent, and W. Losert
J. Cell Sci. 123, 1724-1731 (15 May 2010)

FROUNT Is a Common Regulator of CCR2 and CCR5 Signaling to Control Directional Migration
E. Toda, Y. Terashima, T. Sato, K. Hirose, S. Kanegasaki, and K. Matsushima
J. Immunol. 183, 6387-6394 (15 November 2009)

Collective cell migration requires vesicular trafficking for chemoattractant delivery at the trailing edge
P. W. Kriebel, V. A. Barr, E. C. Rericha, G. Zhang, and C. A. Parent
JCB 183, 949-961 (1 December 2008)

A Protein with Similarity to PTEN Regulates Aggregation Territory Size by Decreasing Cyclic AMP Pulse Size during Dictyostelium discoideum Development
Y. Tang, and R. H. Gomer
Eukaryot Cell 7, 1758-1770 (1 October 2008)

Rap1 Activation in Response to cAMP Occurs Downstream of Ras Activation during Dictyostelium Aggregation
P. Bolourani, G. B. Spiegelman, and G. Weeks
J Biol Chem 283, 10232-10240 (18 April 2008)

Role of Phosphatidylinositol 3-Kinases in Chemotaxis in Dictyostelium
K. Takeda, A. T. Sasaki, H. Ha, H.-A Seung, and R. A. Firtel
J Biol Chem 282, 11874-11884 (20 April 2007)

The Trypanosoma brucei cAMP phosphodiesterases TbrPDEB1 and TbrPDEB2: flagellar enzymes that are essential for parasite virulence
M. Oberholzer, G. Marti, M. Baresic, S. Kunz, A. Hemphill, and T. Seebeck
FASEB J. 21, 720-731 (1 March 2007)

Asymmetric Localization of Calpain 2 during Neutrophil Chemotaxis
P. A. Nuzzi, M. A. Senetar, and A. Huttenlocher
Mol. Biol. Cell 18, 795-805 (1 March 2007)

S-adenosylhomocysteine hydrolase is localized at the front of chemotaxing cells, suggesting a role for transmethylation during migration
S. Shu, D. C. Mahadeo, X. Liu, W. Liu, C. A. Parent, and E. D. Korn
Proc. Natl. Acad. Sci. USA 103, 19788-19793 (26 December 2006)

Nonadaptive Regulation of ERK2 in Dictyostelium: Implications for Mechanisms of cAMP Relay
J. A. Brzostowski, and A. R. Kimmel
Mol. Biol. Cell 17, 4220-4227 (1 October 2006)

Cellular asymmetry and individuality in directional sensing
A. Samadani, J. Mettetal, and A. van Oudenaarden
Proc. Natl. Acad. Sci. USA 103, 11549-11554 (1 August 2006)

Single-molecule analysis of chemoattractant-stimulated membrane recruitment of a PH-domain-containing protein
S. Matsuoka, M. Iijima, T. M. Watanabe, H. Kuwayama, T. Yanagida, P. N. Devreotes, and M. Ueda
J. Cell Sci. 119, 1071-1079 (15 March 2006)

dictyBase, the model organism database for Dictyostelium discoideum
R. L. Chisholm, P. Gaudet, E. M. Just, K. E. Pilcher, P. Fey, S. N. Merchant, and W. A. Kibbe
Nucleic Acids Res 34, D423-D427 (1 January 2006)

Microarray phenotyping in Dictyostelium reveals a regulon of chemotaxis genes
E. O. Booth, N. V. Driessche, O. Zhuchenko, A. Kuspa, and G. Shaulsky
Bioinformatics 21, 4371-4377 (15 December 2005)

Loss of SMEK, a Novel, Conserved Protein, Suppresses mek1 Null Cell Polarity, Chemotaxis, and Gene Expression Defects
M. C. Mendoza, F. Du, N. Iranfar, N. Tang, H. Ma, W. F. Loomis, and R. A. Firtel
Mol. Cell. Biol. 25, 7839-7853 (1 September 2005)

Controlling Cell Behavior Electrically: Current Views and Future Potential
C. D. McCaig, A. M. Rajnicek, B. Song, and M. Zhao
Physiol. Rev. 85, 943-978 (1 July 2005)

Cyclic AMP mediates keratinocyte directional migration in an electric field
C. E. Pullar, and R. R. Isseroff
J. Cell Sci. 118, 2023-2034 (1 May 2005)

GCR1 Can Act Independently of Heterotrimeric G-Protein in Response to Brassinosteroids and Gibberellins in Arabidopsis Seed Germination
J.-G. Chen, S. Pandey, J. Huang, J. M. Alonso, J. R. Ecker, S. M. Assmann, and A. M. Jones
Plant Physiol. 135, 907-915 (1 June 2004)

A G{alpha}-dependent pathway that antagonizes multiple chemoattractant responses that regulate directional cell movement
J. A. Brzostowski, C. A. Parent, and A. R. Kimmel
Genes Dev. 18, 805-815 (1 April 2004)

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