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Vesicle Endocytosis Requires Dynamin-Dependent GTP Hydrolysis at a Fast CNS Synapse

Science, 7 January 2005
Vol. 307, Issue 5706, p. 124-127
DOI: 10.1126/science.1103631

Vesicle Endocytosis Requires Dynamin-Dependent GTP Hydrolysis at a Fast CNS Synapse

  1. Takayuki Yamashita,
  2. Toshihide Hige,
  3. Tomoyuki Takahashi*
  1. Department of Neurophysiology, University of Tokyo Graduate School of Medicine, Tokyo 113-0033, Japan.
  1. * To whom correspondence should be addressed. E-mail: ttakahas-tky{at}


Molecular dependence of vesicular endocytosis was investigated with capacitance measurements at the calyx of Held terminal in brainstem slices. Intraterminal loading of botulinum toxin E revealed that the rapid capacitance transient implicated as “kiss-and-run” was unrelated to transmitter release. The release-related capacitance change decayed with an endocytotic time constant of 10 to 25 seconds, depending on the magnitude of exocytosis. Presynaptic loading of the nonhydrolyzable guanosine 5′-triphosphate (GTP) analog GTPgS or dynamin-1 proline-rich domain peptide abolished endocytosis. These compounds had no immediate effect on exocytosis, but caused a use-dependent rundown of exocytosis. Thus, the guanosine triphosphatase dynamin-1 is indispensable for vesicle endocytosis at this fast central nervous system (CNS) synapse.

  • Received for publication 3 August 2004.
  • Accepted for publication 10 November 2004.


T. Yamashita, T. Hige, and T. Takahashi, Vesicle Endocytosis Requires Dynamin-Dependent GTP Hydrolysis at a Fast CNS Synapse. Science 307, 124-127 (2005).

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Highlights from the Literature
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