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TLR11 Activation of Dendritic Cells by a Protozoan Profilin-Like Protein

Science, 10 June 2005
Vol. 308, Issue 5728, p. 1626-1629
DOI: 10.1126/science.1109893

TLR11 Activation of Dendritic Cells by a Protozoan Profilin-Like Protein

  1. Felix Yarovinsky1,*,
  2. Dekai Zhang3,
  3. John F. Andersen2,
  4. Gerard L. Bannenberg4,,
  5. Charles N. Serhan4,
  6. Matthew S. Hayden3,
  7. Sara Hieny1,
  8. Fayyaz S. Sutterwala3,
  9. Richard A. Flavell3,
  10. Sankar Ghosh3,
  11. Alan Sher1,*
  1. 1 Immunobiology Section, Laboratory of Parasitic Diseases; National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
  2. 2 Medical Entomology Section, Laboratory of Malaria and Vector Research; National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
  3. 3 Section of Immunobiology and Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06520, USA.
  4. 4 Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
  1. * To whom correspondence should be addressed. E-mail: asher{at}niaid.nih.gov (A.S.); fyarovinsky{at}niaid.nih.gov (F.Y.)
  • Present address: Departamento Genética Molecular de Plantas, Centro Nacional de Biotecnología, 28049 Madrid, Spain.

Abstract

Mammalian Toll-like receptors (TLRs) play an important role in the innate recognition of pathogens by dendritic cells (DCs). Although TLRs are clearly involved in the detection of bacteria and viruses, relatively little is known about their function in the innate response to eukaryotic microorganisms. Here we identify a profilin-like molecule from the protozoan parasite Toxoplasma gondii that generates a potent interleukin-12 (IL-12) response in murine DCs that is dependent on myeloid differentiation factor 88. T. gondii profilin activates DCs through TLR11 and is the first chemically defined ligand for this TLR. Moreover, TLR11 is required in vivo for parasite-induced IL-12 production and optimal resistance to infection, thereby establishing a role for the receptor in host recognition of protozoan pathogens.

    • Received for publication 18 January 2005.
    • Accepted for publication 4 April 2005.

    Citation:

    F. Yarovinsky, D. Zhang, J. F. Andersen, G. L. Bannenberg, C. N. Serhan, M. S. Hayden, S. Hieny, F. S. Sutterwala, R. A. Flavell, S. Ghosh, and A. Sher, TLR11 Activation of Dendritic Cells by a Protozoan Profilin-Like Protein. Science 308, 1626-1629 (2005).

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    J. C. Kagan, and G. M. Barton
    Cold Spring Harb. Perspect. Biol. 7, a016253-a016253 (1 March 2015)

    Microbial Sensing by Toll-Like Receptors and Intracellular Nucleic Acid Sensors
    S. Pandey, T. Kawai, and S. Akira
    Cold Spring Harb. Perspect. Biol. 7, a016246-a016246 (1 January 2015)

    The Host Response: Toll-Like Receptor Expression in Periprosthetic Tissues as a Biomarker for Deep Joint Infection
    C. Cipriano, A. Maiti, G. Hale, and W. Jiranek
    J Bone Joint Surg Am 96, 1692-1698 (15 October 2014)

    Use of Transgenic Parasites and Host Reporters To Dissect Events That Promote Interleukin-12 Production during Toxoplasmosis
    D. A. Christian, A. A. Koshy, M. A. Reuter, M. R. Betts, J. C. Boothroyd, and C. A. Hunter
    Infect. Immun. 82, 4056-4067 (1 October 2014)

    Immunoproteasomes Shape the Transcriptome and Regulate the Function of Dendritic Cells
    D. A. de Verteuil, A. Rouette, M.-P. Hardy, S. Lavallee, A. Trofimov, E. Gaucher, and C. Perreault
    J. Immunol. 193, 1121-1132 (1 August 2014)

    Complex Immune Cell Interplay in the Gamma Interferon Response during Toxoplasma gondii Infection
    C. R. Sturge, and F. Yarovinsky
    Infect. Immun. 82, 3090-3097 (1 August 2014)

    Intracellular immunity: finding the enemy within--how cells recognize and respond to intracellular pathogens
    J. C. H. Tam, and D. A. Jacques
    J. Leukoc. Biol. 96, 233-244 (1 August 2014)

    GRA25 Is a Novel Virulence Factor of Toxoplasma gondii and Influences the Host Immune Response
    A. J. Shastri, N. D. Marino, M. Franco, M. B. Lodoen, and J. C. Boothroyd
    Infect. Immun. 82, 2595-2605 (1 June 2014)

    Dendritic cell maturation: functional specialization through signaling specificity and transcriptional programming
    M. Dalod, R. Chelbi, B. Malissen, and T. Lawrence
    EMBO J. 33, 1104-1116 (16 May 2014)

    Toxoplasma gondii Upregulates Interleukin-12 To Prevent Plasmodium berghei-Induced Experimental Cerebral Malaria
    E. W. Settles, L. A. Moser, T. H. Harris, and L. J. Knoll
    Infect. Immun. 82, 1343-1353 (1 March 2014)

    Flagellin A Toll-Like Receptor 5 Agonist as an Adjuvant in Chicken Vaccines
    S. K. Gupta, P. Bajwa, R. Deb, M. M. Chellappa, and S. Dey
    CVI 21, 261-270 (1 March 2014)

    Toll-Like Receptor 11-Initiated Innate Immune Response in Male Mouse Germ Cells
    Q. Chen, W. Zhu, Z. Liu, K. Yan, S. Zhao, and D. Han
    Biol. Reprod. 90, 38-38 (1 February 2014)

    NLRP1 Is an Inflammasome Sensor for Toxoplasma gondii
    S. E. Ewald, J. Chavarria-Smith, and J. C. Boothroyd
    Infect. Immun. 82, 460-468 (1 January 2014)

    Toll-Like Receptor 7 Mediates Early Innate Immune Responses to Malaria
    A. Baccarella, M. F. Fontana, E. C. Chen, and C. C. Kim
    Infect. Immun. 81, 4431-4442 (1 December 2013)

    Profilin-1 Haploinsufficiency Protects Against Obesity-Associated Glucose Intolerance and Preserves Adipose Tissue Immune Homeostasis
    G. R. Romeo, M. Pae, D. Eberle, J. Lee, and S. E. Shoelson
    Diabetes 62, 3718-3726 (1 November 2013)

    Cooperation of TLR12 and TLR11 in the IRF8-Dependent IL-12 Response to Toxoplasma gondii Profilin
    M. Raetz, A. Kibardin, C. R. Sturge, R. Pifer, H. Li, E. Burstein, K. Ozato, S. Larin, and F. Yarovinsky
    J. Immunol. 191, 4818-4827 (1 November 2013)

    Immune effects of dietary anethole on Eimeria acervulina infection
    D. K. Kim, H. S. Lillehoj, S. H. Lee, S. I. Jang, M. S. Park, W. Min, E. P. Lillehoj, and D. Bravo
    Poultry Science 92, 2625-2634 (1 October 2013)

    Campylobacter jejuni carbon starvation protein A (CstA) is involved in peptide utilization, motility and agglutination, and has a role in stimulation of dendritic cells
    J. J. Rasmussen, C. S. Vegge, H. Frokiaer, R. M. Howlett, K. A. Krogfelt, D. J. Kelly, and H. Ingmer
    J Med Microbiol 62, 1135-1143 (1 August 2013)

    TLR-independent neutrophil-derived IFN-{gamma} is important for host resistance to intracellular pathogens
    C. R. Sturge, A. Benson, M. Raetz, C. L. Wilhelm, J. Mirpuri, E. S. Vitetta, and F. Yarovinsky
    Proc. Natl. Acad. Sci. USA 110, 10711-10716 (25 June 2013)

    Immune-neural connections: how the immune system's response to infectious agents influences behavior
    R. H. McCusker, and K. W. Kelley
    J. Exp. Biol. 216, 84-98 (1 January 2013)

    Association of a NOD2 Gene Polymorphism and T-Helper 17 Cells With Presumed Ocular Toxoplasmosis
    M. S. Dutra, S. R. Bela, A. L. Peixoto-Rangel, M. Fakiola, A. G. Cruz, A. Gazzinelli, H. F. Quites, L. M. G. Bahia-Oliveira, R. G. Peixe, W. R. Campos et al.
    The Journal of Infectious Disease 207, 152-163 (1 January 2013)

    UNC93B1 mediates differential trafficking of endosomal TLRs
    B. L. Lee, J. E. Moon, J. H. Shu, L. Yuan, Z. R. Newman, R. Schekman, and G. M. Barton
    elife 2, e00291-e00291 (1 January 2013)

    Toll-like Receptor 11 (TLR11) Prevents Salmonella Penetration into the Murine Peyer Patches
    Z. Shi, Z. Cai, J. Yu, T. Zhang, S. Zhao, E. Smeds, Q. Zhang, F. Wang, C. Zhao, S. Fu et al.
    J Biol Chem 287, 43417-43423 (21 December 2012)

    Impaired Innate Immunity in Mice Deficient in Interleukin-1 Receptor-Associated Kinase 4 Leads to Defective Type 1 T Cell Responses, B Cell Expansion, and Enhanced Susceptibility to Infection with Toxoplasma gondii
    S. R. Bela, M. S. Dutra, E. Mui, A. Montpetit, F. S. Oliveira, S. C. Oliveira, R. M. E. Arantes, L. R. Antonelli, R. McLeod, R. T. Gazzinelli et al.
    Infect. Immun. 80, 4298-4308 (1 December 2012)

    TLR15 Is Unique to Avian and Reptilian Lineages and Recognizes a Yeast-Derived Agonist
    A. C. Boyd, M. Y. Peroval, J. A. Hammond, M. D. Prickett, J. R. Young, and A. L. Smith
    J. Immunol. 189, 4930-4938 (15 November 2012)

    Toll-like receptors in the host defense against Pseudomonas aeruginosa respiratory infection and cystic fibrosis
    S. M. McIsaac, A. W. Stadnyk, and T.-J. Lin
    J. Leukoc. Biol. 92, 977-985 (1 November 2012)

    Trefoil Factor 2 Negatively Regulates Type 1 Immunity against Toxoplasma gondii
    C. McBerry, C. E. Egan, R. Rani, Y. Yang, D. Wu, N. Boespflug, L. Boon, B. Butcher, J. Mirpuri, S. P. Hogan et al.
    J. Immunol. 189, 3078-3084 (15 September 2012)

    TLR9 and MyD88 Are Crucial for the Development of Protective Immunity to Malaria
    N. M. Gowda, X. Wu, and D. C. Gowda
    J. Immunol. 188, 5073-5085 (15 May 2012)

    The Asp299Gly Polymorphism Alters TLR4 Signaling by Interfering with Recruitment of MyD88 and TRIF
    L. Figueroa, Y. Xiong, C. Song, W. Piao, S. N. Vogel, and A. E. Medvedev
    J. Immunol. 188, 4506-4515 (1 May 2012)

    Enzyme-Linked Immunosorbent Assays Based on Neospora caninum Dense Granule Protein 7 and Profilin for Estimating the Stage of Neosporosis
    J. Hiasa, M. Nishimura, K. Itamoto, X. Xuan, H. Inokuma, and Y. Nishikawa
    CVI 19, 411-417 (1 March 2012)

    An Inside Job: Hacking into Janus Kinase/Signal Transducer and Activator of Transcription Signaling Cascades by the Intracellular Protozoan Toxoplasma gondii
    E. Y. Denkers, D. J. Bzik, B. A. Fox, and B. A. Butcher
    Infect. Immun. 80, 476-482 (1 February 2012)

    Sequence specific detection of bacterial 23S ribosomal RNA by TLR13
    X.-D. Li, and Z. J. Chen
    elife 1, e00102-e00102 (1 January 2012)

    Identification and Immunological Characterization of Three Potential Vaccinogens against Cryptosporidium Species
    P. A. Manque, F. Tenjo, U. Woehlbier, A. M. Lara, M. G. Serrano, P. Xu, J. M. Alves, R. B. Smeltz, D. H. Conrad, G. A. Buck et al.
    CVI 18, 1796-1802 (1 November 2011)

    Toxoplasma gondii Induces B7-2 Expression through Activation of JNK Signal Transduction
    P. Morgado, Y.-C. Ong, J. C. Boothroyd, and M. B. Lodoen
    Infect. Immun. 79, 4401-4412 (1 November 2011)

    Toll-like receptors in health and disease in the brain: mechanisms and therapeutic potential
    M. L. Hanke, and T. Kielian
    Clin. Sci. 121, 367-387 (1 November 2011)

    Increased Disease Severity of Parasite-Infected TLR2-/- Mice Is Correlated with Decreased Central Nervous System Inflammation and Reduced Numbers of Cells with Alternatively Activated Macrophage Phenotypes in a Murine Model of Neurocysticercosis
    U. M. Gundra, B. B. Mishra, K. Wong, and J. M. Teale
    Infect. Immun. 79, 2586-2596 (1 July 2011)

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    K.-H. Lee, Y.-J. Liu, A. Biswas, C. Ogawa, and K. S. Kobayashi
    J Biol Chem 286, 5727-5735 (18 February 2011)

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    Z. Shi, Z. Cai, A. Sanchez, T. Zhang, S. Wen, J. Wang, J. Yang, S. Fu, and D. Zhang
    J Biol Chem 286, 4517-4524 (11 February 2011)

    UNC93B1 Is Essential for TLR11 Activation and IL-12-dependent Host Resistance to Toxoplasma gondii
    R. Pifer, A. Benson, C. R. Sturge, and F. Yarovinsky
    J Biol Chem 286, 3307-3314 (4 February 2011)

    Critical coordination of innate immune defense against Toxoplasma gondii by dendritic cells responding via their Toll-like receptors
    B. Hou, A. Benson, L. Kuzmich, A. L. DeFranco, and F. Yarovinsky
    Proc. Natl. Acad. Sci. USA 108, 278-283 (4 January 2011)

    Dendritic Cell Activation Prevents MHC Class II Ubiquitination and Promotes MHC Class II Survival Regardless of the Activation Stimulus
    E. Walseng, K. Furuta, R. S. Goldszmid, K. A. Weih, A. Sher, and P. A. Roche
    J Biol Chem 285, 41749-41754 (31 December 2010)

    Mouse CD8{alpha}+ DCs and human BDCA3+ DCs are major producers of IFN-{lambda} in response to poly IC
    H. Lauterbach, B. Bathke, S. Gilles, C. Traidl-Hoffmann, C. A. Luber, G. Fejer, M. A. Freudenberg, G. M. Davey, D. Vremec, A. Kallies et al.
    JEM 207, 2703-2717 (22 November 2010)

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    Y. Liu, M. Li, S. Fan, Y. Lin, B. Lin, F. Luo, C. Zhang, S. Chen, Y. Li, A. Xu et al.
    J. Immunol. 185, 3391-3400 (15 September 2010)

    Toxoplasma gondii Cyclophilin 18 Regulates the Proliferation and Migration of Murine Macrophages and Spleen Cells
    H. M. Ibrahim, X. Xuan, and Y. Nishikawa
    CVI 17, 1322-1329 (1 September 2010)

    CX3CR1+ CD8{alpha}+ dendritic cells are a steady-state population related to plasmacytoid dendritic cells
    L. Bar-On, T. Birnberg, K. L. Lewis, B. T. Edelson, D. Bruder, K. Hildner, J. Buer, K. M. Murphy, B. Reizis, S. Jung et al.
    Proc. Natl. Acad. Sci. USA 107, 14745-14750 (17 August 2010)

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    A. M. Keestra, M. R. de Zoete, L. I. Bouwman, and J. P. M. van Putten
    J. Immunol. 185, 460-467 (1 July 2010)

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    S. F. G. van Helden, K. van den Dries, M. M. Oud, R. A. P. Raymakers, M. G. Netea, F. N. van Leeuwen, and C. G. Figdor
    J. Immunol. 184, 1280-1291 (1 February 2010)

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    K. E. Sivick, and H. L. T. Mobley
    Infect. Immun. 78, 568-585 (1 February 2010)

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    J. Y. Lee, L. Zhao, and D. H. Hwang
    Nutrition Reviews 68, 38-61 (1 January 2010)

    Early Response of Mucosal Epithelial Cells during Toxoplasma gondii Infection
    C.-H. Ju, A. Chockalingam, and C. A. Leifer
    J. Immunol. 183, 7420-7427 (1 December 2009)

    Transcriptional Regulation of Tlr11 Gene Expression in Epithelial Cells
    Z. Cai, Z. Shi, A. Sanchez, T. Zhang, M. Liu, J. Yang, F. Wang, and D. Zhang
    J Biol Chem 284, 33088-33096 (27 November 2009)

    A single polymorphic amino acid on Toxoplasma gondii kinase ROP16 determines the direct and strain-specific activation of Stat3
    M. Yamamoto, D. M. Standley, S. Takashima, H. Saiga, M. Okuyama, H. Kayama, E. Kubo, H. Ito, M. Takaura, T. Matsuda et al.
    JEM 206, 2747-2760 (23 November 2009)

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    D. Wang, and D.-J. Jang
    Cancer Res. 69, 8200-8207 (15 October 2009)

    Toxoplasma gondii Cyclophilin 18-Mediated Production of Nitric Oxide Induces Bradyzoite Conversion in a CCR5-Dependent Manner
    H. M. Ibrahim, H. Bannai, X. Xuan, and Y. Nishikawa
    Infect. Immun. 77, 3686-3695 (1 September 2009)

    Transcriptional Regulation of the Novel Toll-like Receptor Tlr13
    Z. Shi, Z. Cai, S. Wen, C. Chen, C. Gendron, A. Sanchez, K. Patterson, S. Fu, J. Yang, D. Wildman et al.
    J Biol Chem 284, 20540-20547 (31 July 2009)

    The origins of apicomplexan sequence innovation
    J. Wasmuth, J. Daub, J. M. Peregrin-Alvarez, C. A.M. Finney, and J. Parkinson
    Genome Res 19, 1202-1213 (1 July 2009)

    Pathogen recognition in the innate immune response
    H. Kumar, T. Kawai, and S. Akira
    Biochem. J. 420, 1-16 (15 May 2009)

    The roles of TLRs, RLRs and NLRs in pathogen recognition
    T. Kawai, and S. Akira
    Int Immunol 21, 317-337 (1 April 2009)

    Recombinant Ov-ASP-1, a Th1-Biased Protein Adjuvant Derived from the Helminth Onchocerca volvulus, Can Directly Bind and Activate Antigen-Presenting Cells
    Y. He, S. J. Barker, A. J. MacDonald, Y. Yu, L. Cao, J. Li, R. Parhar, S. Heck, S. Hartmann, D. T. Golenbock et al.
    J. Immunol. 182, 4005-4016 (1 April 2009)

    Pathogen Recognition and Inflammatory Signaling in Innate Immune Defenses
    T. H. Mogensen, S. J. Barker, A. J. MacDonald, Y. Yu, L. Cao, J. Li, R. Parhar, S. Heck, S. Hartmann, D. T. Golenbock et al.
    Clin. Microbiol. Rev. 22, 240-273 (1 April 2009)

    TLRs and innate immunity
    B. A. Beutler, S. J. Barker, A. J. MacDonald, Y. Yu, L. Cao, J. Li, R. Parhar, S. Heck, S. Hartmann, D. T. Golenbock et al.
    Blood 113, 1399-1407 (12 February 2009)

    Phosphoinositide 3-Kinase-Dependent Inhibition of Dendritic Cell Interleukin-12 Production by Giardia lamblia
    J. D. Kamda, and S. M. Singer
    Infect. Immun. 77, 685-693 (1 February 2009)

    Toxoplasma gondii Prevents Chromatin Remodeling Initiated by TLR-Triggered Macrophage Activation
    J. Leng, B. A. Butcher, C. E. Egan, D. S. Abi Abdallah, and E. Y. Denkers
    J. Immunol. 182, 489-497 (1 January 2009)

    Recognition of Toxoplasma gondii by TLR11 Prevents Parasite-Induced Immunopathology
    F. Yarovinsky, S. Hieny, and A. Sher
    J. Immunol. 181, 8478-8484 (15 December 2008)

    Dendritic Cells Expressing Plasmacytoid Marker PDCA-1 Are Trojan Horses during Toxoplasma gondii Infection
    A. L. Bierly, W. J. Shufesky, W. Sukhumavasi, A. E. Morelli, and E. Y. Denkers
    J. Immunol. 181, 8485-8491 (15 December 2008)

    TLR Adaptor MyD88 Is Essential for Pathogen Control during Oral Toxoplasma gondii Infection but Not Adaptive Immunity Induced by a Vaccine Strain of the Parasite
    W. Sukhumavasi, C. E. Egan, A. L. Warren, G. A. Taylor, B. A. Fox, D. J. Bzik, and E. Y. Denkers
    J. Immunol. 181, 3464-3473 (1 September 2008)

    Phase I Study and Preliminary Pharmacology of the Novel Innate Immune Modulator rBBX-01 in Gynecologic Cancers
    J. S. Rader, C. F. Aylsworth, D. A. Juckett, D. G. Mutch, M. A. Powell, L. Lippmann, and N. V. Dimitrov
    Clin. Cancer Res. 14, 3089-3097 (15 May 2008)

    Plasmacytoid Dendritic Cells Are Activated by Toxoplasma gondii to Present Antigen and Produce Cytokines
    M. Pepper, F. Dzierszinski, E. Wilson, E. Tait, Q. Fang, F. Yarovinsky, T. M. Laufer, D. Roos, and C. A. Hunter
    J. Immunol. 180, 6229-6236 (1 May 2008)

    T cell expression of MyD88 is required for resistance to Toxoplasma gondii
    D. F. LaRosa, J. S. Stumhofer, A. E. Gelman, A. H. Rahman, D. K. Taylor, C. A. Hunter, and L. A. Turka
    Proc. Natl. Acad. Sci. USA 105, 3855-3860 (11 March 2008)

    Review Article: Expression and Function of Toll-Like Receptors at the Maternal--Fetal Interface
    K. Koga, and G. Mor
    Reproductive Sciences 15, 231-242 (1 March 2008)

    Malaria Parasites Require TLR9 Signaling for Immune Evasion by Activating Regulatory T Cells
    H. Hisaeda, K. Tetsutani, T. Imai, C. Moriya, L. Tu, S. Hamano, X. Duan, B. Chou, H. Ishida, A. Aramaki et al.
    J. Immunol. 180, 2496-2503 (15 February 2008)

    Tyrosine Phosphorylation of MyD88 Adapter-like (Mal) Is Critical for Signal Transduction and Blocked in Endotoxin Tolerance
    W. Piao, C. Song, H. Chen, L. M. Wahl, K. A. Fitzgerald, L. A. O'Neill, and A. E. Medvedev
    J Biol Chem 283, 3109-3119 (8 February 2008)

    Phosphoinositide-3-Kinase-Dependent, MyD88-Independent Induction of CC-Type Chemokines Characterizes the Macrophage Response to Toxoplasma gondii Strains with High Virulence
    C. W. Lee, W. Sukhumavasi, and E. Y. Denkers
    Infect. Immun. 75, 5788-5797 (1 December 2007)

    Exosomes released from macrophages infected with intracellular pathogens stimulate a proinflammatory response in vitro and in vivo
    S. Bhatnagar, K. Shinagawa, F. J. Castellino, and J. S. Schorey
    Blood 110, 3234-3244 (1 November 2007)

    Salmonella Flagellin Induces Bystander Activation of Splenic Dendritic Cells and Hinders Bacterial Replication In Vivo
    R.-M. Salazar-Gonzalez, A. Srinivasan, A. Griffin, G. Muralimohan, J. M. Ertelt, R. Ravindran, A. T. Vella, and S. J. McSorley
    J. Immunol. 179, 6169-6175 (1 November 2007)

    Mouse Neutrophils Require JNK2 MAPK for Toxoplasma gondii-Induced IL-12p40 and CCL2/MCP-1 Release
    W. Sukhumavasi, C. E. Egan, and E. Y. Denkers
    J. Immunol. 179, 3570-3577 (15 September 2007)

    Activation of TLR2 and TLR4 by Glycosylphosphatidylinositols Derived from Toxoplasma gondii
    F. Debierre-Grockiego, M. A. Campos, N. Azzouz, J. Schmidt, U. Bieker, M. G. Resende, D. S. Mansur, R. Weingart, R. R. Schmidt, D. T. Golenbock et al.
    J. Immunol. 179, 1129-1137 (15 July 2007)

    TLR Agonists Selectively Promote Terminal Plasma Cell Differentiation of B Cell Subsets Specialized in Thymus-Independent Responses
    L. Genestier, M. Taillardet, P. Mondiere, H. Gheit, C. Bella, and T. Defrance
    J. Immunol. 178, 7779-7786 (15 June 2007)

    Diacylglycerol kinase {zeta} regulates microbial recognition and host resistance to Toxoplasma gondii
    C.-H. Liu, F. S. Machado, R. Guo, K. E. Nichols, A. W. Burks, J. C. Aliberti, and X.-P. Zhong
    JEM 204, 781-792 (16 April 2007)

    Gadd45{alpha} Regulates p38-Dependent Dendritic Cell Cytokine Production and Th1 Differentiation
    L. Jirmanova, D. Jankovic, A. J. Fornace, and J. D. Ashwell
    J. Immunol. 178, 4153-4158 (1 April 2007)

    Immunostimulatory RNA is a potent inducer of antigen-specific cytotoxic and humoral immune response in vivo
    S. Hamm, A. Heit, M. Koffler, K. M. Huster, S. Akira, D. H. Busch, H. Wagner, and S. Bauer
    Int Immunol 19, 297-304 (1 March 2007)

    Chlamydia muridarum Infection Elicits a Beta Interferon Response in Murine Oviduct Epithelial Cells Dependent on Interferon Regulatory Factor 3 and TRIF
    W. A. Derbigny, S.-C. Hong, M. S. Kerr, M. Temkit, and R. M. Johnson
    Infect. Immun. 75, 1280-1290 (1 March 2007)

    Menstrual cycle-dependent changes of Toll-like receptors in endometrium
    R. Aflatoonian, E. Tuckerman, S.L. Elliott, C. Bruce, A. Aflatoonian, T.C. Li, and A. Fazeli
    Hum Reprod 22, 586-593 (1 February 2007)

    Review: Pathogen-associated molecular pattern contamination as putative endogenous ligands of Toll-like receptors
    M.-F. Tsan, and Baochong Gao
    Innate Immunity 13, 6-14 (1 February 2007)

    Role of Toll-like receptor 4 in innate immune responses in a mouse model of acute otitis media
    T. Hirano, S. Kodama, K. Fujita, K. Maeda, and M. Suzuki
    FEMS Immunol Med Microbiol 49, 75-83 (1 February 2007)

    Ikaros is required for plasmacytoid dendritic cell differentiation
    D. Allman, M. Dalod, C. Asselin-Paturel, T. Delale, S. H. Robbins, G. Trinchieri, C. A. Biron, P. Kastner, and S. Chan
    Blood 108, 4025-4034 (15 December 2006)

    Human monocyte-derived dendritic cells express TLR9 and react directly to the CpG-A oligonucleotide D19
    V. Hoene, M. Peiser, and R. Wanner
    J. Leukoc. Biol. 80, 1328-1336 (1 December 2006)

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    K. S. Masek, J. Fiore, M. Leitges, S.-F. Yan, B. D. Freedman, and C. A. Hunter
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