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Tissue Geometry Determines Sites of Mammary Branching Morphogenesis in Organotypic Cultures

Science, 13 October 2006
Vol. 314, Issue 5797, p. 298-300
DOI: 10.1126/science.1131000

Tissue Geometry Determines Sites of Mammary Branching Morphogenesis in Organotypic Cultures

  1. Celeste M. Nelson1,*,
  2. Martijn M. VanDuijn3,
  3. Jamie L. Inman1,
  4. Daniel A. Fletcher2,3,
  5. Mina J. Bissell1,*
  1. 1 Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
  2. 2 Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
  3. 3 Department of Bioengineering, University of California, Berkeley, CA 94720, USA.
  1. * To whom correspondence should be addressed. E-mail: mjbissell{at}lbl.gov (M.J.B.); cmnelson{at}lbl.gov (C.M.N.)

Abstract

The treelike structures of many organs, including the mammary gland, are generated by branching morphogenesis, a reiterative process of branch initiation and invasion from a preexisting epithelium. Using a micropatterning approach to control the initial three-dimensional structure of mouse mammary epithelial tubules in culture, combined with an algorithm to quantify the extent of branching, we found that the geometry of tubules dictates the position of branches. We predicted numerically and confirm experimentally that branches initiate at sites with a local minimum in the concentration of autocrine inhibitory morphogens, such as transforming growth factor–β. These results reveal that tissue geometry can control organ morphogenesis by defining the local cellular microenvironment, a finding that has relevance to control of invasion and metastasis.

  • Received for publication 7 June 2006.
  • Accepted for publication 6 September 2006.

Citation:

C. M. Nelson, M. M. VanDuijn, J. L. Inman, D. A. Fletcher, and M. J. Bissell, Tissue Geometry Determines Sites of Mammary Branching Morphogenesis in Organotypic Cultures. Science 314, 298-300 (2006).

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