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Differential Transmission of Actin Motion Within Focal Adhesions

Science, 5 January 2007
Vol. 315, Issue 5808, p. 111-115
DOI: 10.1126/science.1135085

Differential Transmission of Actin Motion Within Focal Adhesions

  1. Ke Hu*,
  2. Lin Ji*,
  3. Kathryn T. Applegate,
  4. Gaudenz Danuser,
  5. Clare M. Waterman-Storer
  1. Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
  1. To whom correspondence should be addressed. E-mail: waterman{at}scripps.edu (C.M.W.-S.); gdanuser{at}scripps.edu (G.D.)
  • * These authors contributed equally to this work.

Abstract

Cell migration requires the transmission of motion generated in the actin cytoskeleton to the extracellular environment through a complex assembly of proteins in focal adhesions. We developed correlational fluorescent speckle microscopy to measure the coupling of focal-adhesion proteins to actin filaments. Different classes of focal-adhesion structural and regulatory molecules exhibited varying degrees of correlated motions with actin filaments, indicating hierarchical transmission of actin motion through focal adhesions. Interactions between vinculin, talin, and actin filaments appear to constitute a slippage interface between the cytoskeleton and integrins, generating a molecular clutch that is regulated during the morphodynamic transitions of cell migration.

    • Received for publication 13 September 2006.
    • Accepted for publication 16 November 2006.

    Citation:

    K. Hu, L. Ji, K. T. Applegate, G. Danuser, and C. M. Waterman-Storer, Differential Transmission of Actin Motion Within Focal Adhesions. Science 315, 111-115 (2007).

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