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Bora and the Kinase Aurora A Cooperatively Activate the Kinase Plk1 and Control Mitotic Entry

Science, 20 June 2008
Vol. 320, Issue 5883, p. 1655-1658
DOI: 10.1126/science.1157425

Bora and the Kinase Aurora A Cooperatively Activate the Kinase Plk1 and Control Mitotic Entry

  1. Akiko Seki1,
  2. Judith A. Coppinger2,
  3. Chang-Young Jang1,
  4. John R. Yates, III2,
  5. Guowei Fang1,*
  1. 1 Department of Biological Sciences, Stanford University, Stanford, CA 94305–5020, USA.
  2. 2 Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
  1. * To whom correspondence should be addressed. E-mail: gwfang{at}stanford.edu

Abstract

A central question in the study of cell proliferation is, what controls cell-cycle transitions? Although the accumulation of mitotic cyclins drives the transition from the G2 phase to the M phase in embryonic cells, the trigger for mitotic entry in somatic cells remains unknown. We report that the synergistic action of Bora and the kinase Aurora A (Aur-A) controls the G2-M transition. Bora accumulates in the G2 phase and promotes Aur-A–mediated activation of Polo-like kinase 1 (Plk1), leading to the activation of cyclin-dependent kinase 1 and mitotic entry. Mechanistically, Bora interacts with Plk1 and controls the accessibility of its activation loop for phosphorylation and activation by Aur-A. Thus, Bora and Aur-A control mitotic entry, which provides a mechanism for one of the most important yet ill-defined events in the cell cycle.

  • Received for publication 6 March 2008.
  • Accepted for publication 21 May 2008.

Citation:

A. Seki, J. A. Coppinger, C.-Y. Jang, J. R. Yates, and G. Fang, Bora and the Kinase Aurora A Cooperatively Activate the Kinase Plk1 and Control Mitotic Entry. Science 320, 1655-1658 (2008).

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