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Orc1 Controls Centriole and Centrosome Copy Number in Human Cells

Science, 6 February 2009
Vol. 323, Issue 5915, p. 789-793
DOI: 10.1126/science.1166745

Orc1 Controls Centriole and Centrosome Copy Number in Human Cells

  1. Adriana S. Hemerly1,2,
  2. Supriya G. Prasanth1,*,
  3. Khalid Siddiqui1,,
  4. Bruce Stillman1,
  1. 1 Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor 11724, NY, USA.
  2. 2 Instituto de Bioquímica Médica, UFRJ, 21941-590, Rio de Janeiro, Brazil.
  1. To whom correspondence should be addressed. E-mail: stillman{at}cshl.edu
  • * Present address: Department of Cell and Developmental Biology, 601 South Goodwin Avenue, University of Illinois at Urbana–Champaign, Champaign, IL 61801, USA.

  • Present address: Clare Hall Laboratories, Cancer Research UK, South Mimms, Herts, EN6 3LD, UK.

Abstract

Centrosomes, each containing a pair of centrioles, organize microtubules in animal cells, particularly during mitosis. DNA and centrosomes are normally duplicated once before cell division to maintain optimal genome integrity. We report a new role for the Orc1 protein, a subunit of the origin recognition complex (ORC) that is a key component of the DNA replication licensing machinery, in controlling centriole and centrosome copy number in human cells, independent of its role in DNA replication. Cyclin A promotes Orc1 localization to centrosomes where Orc1 prevents Cyclin E-dependent reduplication of both centrioles and centrosomes in a single cell division cycle. The data suggest that Orc1 is a regulator of centriole and centrosome reduplication as well as the initiation of DNA replication.

    • Received for publication 2 October 2008.
    • Accepted for publication 9 December 2008.

    Citation:

    A. S. Hemerly, S. G. Prasanth, K. Siddiqui, and B. Stillman, Orc1 Controls Centriole and Centrosome Copy Number in Human Cells. Science 323, 789-793 (2009).

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