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Structural Mechanism of Abscisic Acid Binding and Signaling by Dimeric PYR1

Science, 4 December 2009
Vol. 326, Issue 5958, p. 1373-1379
DOI: 10.1126/science.1181829

Structural Mechanism of Abscisic Acid Binding and Signaling by Dimeric PYR1

  1. Noriyuki Nishimura1,*,
  2. Kenichi Hitomi2,3,*,
  3. Andrew S. Arvai2,*,
  4. Robert P. Rambo3,*,
  5. Chiharu Hitomi2,
  6. Sean R. Cutler4,
  7. Julian I. Schroeder1,
  8. Elizabeth D. Getzoff2,
  1. 1Division of Biological Sciences, Cell and Developmental Biology Section, University of California at San Diego, La Jolla, CA 92093, USA.
  2. 2Department of Molecular Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
  3. 3Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
  4. 4Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California at Riverside, Riverside, CA 92521, USA.
  1. To whom correspondence should be addressed. E-mail: edg{at}scripps.edu

Abstract

The phytohormone abscisic acid (ABA) acts in seed dormancy, plant development, drought tolerance, and adaptive responses to environmental stresses. Structural mechanisms mediating ABA receptor recognition and signaling remain unknown but are essential for understanding and manipulating abiotic stress resistance. Here, we report structures of pyrabactin resistance 1 (PYR1), a prototypical PYR/PYR1-like (PYL)/regulatory component of ABA receptor (RCAR) protein that functions in early ABA signaling. The crystallographic structure reveals an α/β helix–grip fold and homodimeric assembly, verified in vivo by coimmunoprecipitation. ABA binding within a large internal cavity switches structural motifs distinguishing ABA-free “open-lid” from ABA-bound “closed-lid” conformations. Small-angle x-ray scattering suggests that ABA signals by converting PYR1 to a more compact, symmetric closed-lid dimer. Site-directed PYR1 mutants designed to disrupt hormone binding lose ABA-triggered interactions with type 2C protein phosphatase partners in planta.

  • * These authors contributed equally to this work.

  • Received for publication 11 September 2009.
  • Accepted for publication 9 October 2009.

Citation:

N. Nishimura, K. Hitomi, A. S. Arvai, R. P. Rambo, C. Hitomi, S. R. Cutler, J. I. Schroeder, and E. D. Getzoff, Structural Mechanism of Abscisic Acid Binding and Signaling by Dimeric PYR1. Science 326, 1373-1379 (2009).

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