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Two Histone Marks Establish the Inner Centromere and Chromosome Bi-Orientation

Science, 8 October 2010
Vol. 330, Issue 6001, p. 239-243
DOI: 10.1126/science.1194498

Two Histone Marks Establish the Inner Centromere and Chromosome Bi-Orientation

  1. Yuya Yamagishi1,2,*,
  2. Takashi Honda1,3,*,
  3. Yuji Tanno1,
  4. Yoshinori Watanabe1,2,3,
  1. 1Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo 113-0032, Japan.
  2. 2Graduate Program in Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan.
  3. 3Graduate School of Agricultural and Life Science, University of Tokyo, Yayoi, Tokyo 113-0032, Japan.
  1. To whom correspondence should be addressed. E-mail: ywatanab{at}iam.u-tokyo.ac.jp
  1. * These authors contributed equally to this work.

Abstract

For proper partitioning of chromosomes in mitosis, the chromosomal passenger complex (CPC) including Aurora B and survivin must be localized at the center of paired kinetochores, at the site called the inner centromere. It is largely unknown what defines the inner centromere and how the CPC is targeted to this site. Here, we show that the phosphorylation of histone H3–threonine 3 (H3-pT3) mediated by Haspin cooperates with Bub1-mediated histone 2A–serine 121 (H2A-S121) phosphorylation in targeting the CPC to the inner centromere in fission yeast and human cells. H3-pT3 promotes nucleosome binding of survivin, whereas phosphorylated H2A-S121 facilitates the binding of shugoshin, the centromeric CPC adaptor. Haspin colocalizes with cohesin by associating with Pds5, whereas Bub1 localizes at kinetochores. Thus, the inner centromere is defined by intersection of two histone kinases.

  • Received for publication 30 June 2010.
  • Accepted for publication 17 August 2010.

Citation:

Y. Yamagishi, T. Honda, Y. Tanno, and Y. Watanabe, Two Histone Marks Establish the Inner Centromere and Chromosome Bi-Orientation. Science 330, 239-243 (2010).

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