RT Journal Article
SR Electronic
T1 Obtaining and Estimating Kinetic Parameters from the Literature
JF Science Signaling
JO Sci. Signal.
FD American Association for the Advancement of Science
SP tr8
OP tr8
DO 10.1126/scisignal.2001988
VO 4
IS 191
A1 Neves, Susana R.
YR 2011
UL http://stke.sciencemag.org/content/4/191/tr8.abstract
AB This Teaching Resource provides lecture notes, slides, and a student assignment for a lecture on strategies for the development of mathematical models. Many biological processes can be represented mathematically as systems of ordinary differential equations (ODEs). Simulations with these mathematical models can provide mechanistic insight into the underlying biology of the system. A prerequisite for running simulations, however, is the identification of kinetic parameters that correspond closely with the biological reality. This lecture presents an overview of the steps required for the development of kinetic ODE models and describes experimental methods that can yield kinetic parameters and concentrations of reactants, which are essential for the development of kinetic models. Strategies are provided to extract necessary parameters from published data. The homework assignment requires students to find parameters appropriate for a well-studied biological regulatory system, convert these parameters into appropriate units, and interpret how different values of these parameters may lead to different biological behaviors.