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Protocols Archive

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Cell Biology
A Dual Array-Based Approach to Assess the Abundance and Posttranslational Modification State of Signaling Proteins
Katrin Luckert, Taranjit S. Gujral, Marina Chan, Thomas O. Joos, Peter K. Sorger, Gavin MacBeath, and Oliver Pötz (10 January 2012)
Sci. Signal. 5 (206), pl1. [DOI: 10.1126/scisignal.2002372]
Combining two strategies enables simultaneous quantification of multiple signaling proteins. Abstract »   Full Text »   PDF »  
Biochemistry
A Method to Separate Nuclear, Cytosolic, and Membrane-Associated Signaling Molecules in Cultured Cells
Xiaoyong Liu and François Fagotto (13 December 2011)
Sci. Signal. 4 (203), pl2. [DOI: 10.1126/scisignal.2002373]
A semipermeabilization step and a specific Percoll gradient composition enable effective cellular fractionation. Abstract »   Full Text »   PDF »  

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