Contents
Vol 1, Issue 34
Contents
Protocol
- Application of Fluorescence Resonance Energy Transfer and Magnetic Twisting Cytometry to Quantify Mechanochemical Signaling Activities in a Living Cell
Get detailed instructions for delivering biologically relevant mechanical stress to individual cells and observing the intracellular signaling activities that ensue.
Presentation
- Defining Drug Targets in Yeast Haploinsufficiency Screens: Application to Human Translational Pharmacology
Identifying targets of drugs in yeast using genome-wide drug-induced haploinsufficiency is a viable approach to predicting drug targets in humans.
Editors' Choice
- A Cryptic Response?
Cryptochrome appears to play a critical role in fruit fly magnetoreception.
- Telling Polys Apart
Antibodies have been developed that differentiate between polyubiquitin chains that target proteins for degradation and those that enhance protein function.
- Becoming Brown Fat
Insights into the differentiation of brown adipocytes may lead to unexplored approaches to treating obesity.
- Sensing Alarm
A mysterious ganglion at the tip of the nose is an olfactory subsystem that senses alarm pheromones in mice.
- Jam the Signal
A small, nontoxic antibiotic candidate interferes with bacterial detection of the host and inhibits infection, in a therapeutic approach that may avoid development of resistance.
- Fixed But Flexible
Variations in component levels of the antigen-induced signaling pathway affect the final response of activated immune cells, conferring flexibility on the system.
- To Divide or Not to Divide
For quiescent cells to periodically divide and then rest, a member of the Notch signaling pathway HES1 must be present; this protein is also activated in some tumors.
