RhoB controls the Rab11-mediated recycling and surface reappearance of LFA-1 in migrating T lymphocytes

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Science Signaling  12 Dec 2017:
Vol. 10, Issue 509, eaai8629
DOI: 10.1126/scisignal.aai8629

Recycling integrins

T cells must rapidly migrate along endothelial cell surfaces and transmigrate to reach sites of infection and inflammation in tissues. The cell surface integrin LFA-1 binds to its ligand ICAM-1 on endothelial cells in a dynamic manner so that T cells rapidly adhere to and detach from the endothelial surface to migrate quickly. Using biochemical and imaging analyses, Samuelsson et al. identified a process by which LFA-1, internalized after its interaction with ICAM-1, is recycled back to the T cell surface to maintain migration. LFA-1 bound to the GTPase RhoB in the cytosol, which mediated its recruitment to Rab11-positive rapidly recycling endosomes. Loss of RhoB function resulted in the cytosolic accumulation of LFA-1 at the rear of the cell, reduced cell surface abundance of LFA-1, and impaired T cell migration.

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