Research ArticleCell Biology

The DUF1669 domain of FAM83 family proteins anchor casein kinase 1 isoforms

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Science Signaling  22 May 2018:
Vol. 11, Issue 531, eaao2341
DOI: 10.1126/scisignal.aao2341

Subcellular targeting of CK1

FAM83 proteins participate in various cellular processes and are characterized by an N-terminal “domain of unknown function” called DUF1669. Fulcher et al. found that FAM83 family members interacted with a specific subset of casein kinase 1 (CK1) isoforms in vitro through the DUF1669 domain. Each of the eight FAM83 family members exhibited a distinct pattern of subcellular localization and colocalized with specific CK1 isoforms in cultured cells. Experiments in which DUF1669 domains were swapped among FAM83 family members suggested that DUF1669 determines the specificity of the FAM83 protein for particular CK1 isoforms. Because CK1 isoforms are thought to be constitutively active protein kinases, the ability of FAM83 proteins to anchor CK1 isoforms may be an important mechanism for targeting CK1 activity to specific subcellular locations and substrates.

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