Manifold roles of β-arrestins in GPCR signaling elucidated with siRNA and CRISPR/Cas9

Louis M. Luttrell; Jialu Wang; Bianca Plouffe; Jeffrey S. Smith; Lama Yamani; Suneet Kaur; Pierre-Yves Jean-Charles; Christophe Gauthier; Mi-Hye Lee; Biswaranjan Pani; Jihee Kim; Seungkirl Ahn; Sudarshan Rajagopal; Eric Reiter; Michel Bouvier; Sudha K. Shenoy; Stéphane A. Laporte; Howard A. Rockman; Robert J. Lefkowitz

doi:10.1126/scisignal.aat7650

Research ArticleGPCR SIGNALING

Manifold roles of β-arrestins in GPCR signaling elucidated with siRNA and CRISPR/Cas9

View ORCID ProfileLouis M. Luttrell ¹,²,³,
Jialu Wang⁴,
View ORCID ProfileBianca Plouffe⁵,
View ORCID ProfileJeffrey S. Smith⁴,⁶,
Lama Yamani⁷,
Suneet Kaur⁴,
Pierre-Yves Jean-Charles⁴,
Christophe Gauthier⁸,
Mi-Hye Lee¹,
View ORCID ProfileBiswaranjan Pani⁴,
Jihee Kim⁴,
Seungkirl Ahn⁴,
View ORCID ProfileSudarshan Rajagopal ⁴,⁶,
Eric Reiter ⁸,
View ORCID ProfileMichel Bouvier ⁵,
View ORCID ProfileSudha K. Shenoy ⁴,⁹,
View ORCID ProfileStéphane A. Laporte ⁷,
Howard A. Rockman ⁴,⁹,¹⁰, and
View ORCID ProfileRobert J. Lefkowitz ⁴,⁶,¹¹,*

¹Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA.
²Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA.
³Research Service of the Ralph H. Johnson Veterans Affairs Medical Center, Charleston, SC 29401, USA.
⁴Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.
⁵Department of Biochemistry and Molecular Medicine, Institute for Research in Immunology and Cancer, University of Montreal, Montreal, Quebec H3C IJ4, Canada.
⁶Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
⁷Department of Medicine, Research Institute of the McGill University Health Center, McGill University, Montreal, Quebec H4A 3J1, Canada.
⁸Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, CNRS, Université de Tours, 37380 Nouzilly, France.
⁹Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.
¹⁰Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA.
¹¹Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA.

↵*Corresponding author. Email: lefko001{at}receptor-biol.duke.edu

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Science Signaling 25 Sep 2018:
Vol. 11, Issue 549, eaat7650
DOI: 10.1126/scisignal.aat7650

Louis M. Luttrell

1Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA.

2Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA.

3Research Service of the Ralph H. Johnson Veterans Affairs Medical Center, Charleston, SC 29401, USA.

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ORCID record for Louis M. Luttrell

Jialu Wang

4Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.

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Bianca Plouffe

5Department of Biochemistry and Molecular Medicine, Institute for Research in Immunology and Cancer, University of Montreal, Montreal, Quebec H3C IJ4, Canada.

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ORCID record for Bianca Plouffe

Jeffrey S. Smith

4Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.

6Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.

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ORCID record for Jeffrey S. Smith

Lama Yamani

7Department of Medicine, Research Institute of the McGill University Health Center, McGill University, Montreal, Quebec H4A 3J1, Canada.

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Suneet Kaur

4Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.

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Pierre-Yves Jean-Charles

4Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.

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Christophe Gauthier

8Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, CNRS, Université de Tours, 37380 Nouzilly, France.

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Mi-Hye Lee

1Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA.

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Biswaranjan Pani

4Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.

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Jihee Kim

4Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.

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Seungkirl Ahn

4Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.

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Sudarshan Rajagopal

4Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.

6Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.

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Eric Reiter

8Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, CNRS, Université de Tours, 37380 Nouzilly, France.

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Michel Bouvier

5Department of Biochemistry and Molecular Medicine, Institute for Research in Immunology and Cancer, University of Montreal, Montreal, Quebec H3C IJ4, Canada.

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Sudha K. Shenoy

4Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.

9Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.

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Stéphane A. Laporte

7Department of Medicine, Research Institute of the McGill University Health Center, McGill University, Montreal, Quebec H4A 3J1, Canada.

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Howard A. Rockman

4Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.

9Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.

10Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA.

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Robert J. Lefkowitz

4Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.

6Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.

11Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA.

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ORCID record for Robert J. Lefkowitz
For correspondence: lefko001@receptor-biol.duke.edu

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vol. 11 no. 549

DOI:

https://doi.org/10.1126/scisignal.aat7650

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Received April 2, 2018
Accepted September 7, 2018
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Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works http://www.sciencemag.org/about/science-licenses-journal-article-reuseThis is an article distributed under the terms of the Science Journals Default License.

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Manifold roles of β-arrestins in GPCR signaling elucidated with siRNA and CRISPR/Cas9

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