Manifold roles of β-arrestins in GPCR signaling elucidated with siRNA and CRISPR/Cas9

Louis M. Luttrell; Jialu Wang; Bianca Plouffe; Jeffrey S. Smith; Lama Yamani; Suneet Kaur; Pierre-Yves Jean-Charles; Christophe Gauthier; Mi-Hye Lee; Biswaranjan Pani; Jihee Kim; Seungkirl Ahn; Sudarshan Rajagopal; Eric Reiter; Michel Bouvier; Sudha K. Shenoy; Stéphane A. Laporte; Howard A. Rockman; Robert J. Lefkowitz

doi:10.1126/scisignal.aat7650

Research ArticleGPCR SIGNALING

Manifold roles of β-arrestins in GPCR signaling elucidated with siRNA and CRISPR/Cas9

Louis M. Luttrell ¹,²,³,
Jialu Wang⁴,
Bianca Plouffe⁵,
Jeffrey S. Smith⁴,⁶,
Lama Yamani⁷,
Suneet Kaur⁴,
Pierre-Yves Jean-Charles⁴,
Christophe Gauthier⁸,
Mi-Hye Lee¹,
Biswaranjan Pani⁴,
Jihee Kim⁴,
Seungkirl Ahn⁴,
Sudarshan Rajagopal ⁴,⁶,
Eric Reiter ⁸,
Michel Bouvier ⁵,
Sudha K. Shenoy ⁴,⁹,
Stéphane A. Laporte ⁷,
Howard A. Rockman ⁴,⁹,¹⁰, and
Robert J. Lefkowitz ⁴,⁶,¹¹,*

¹Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA.
²Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA.
³Research Service of the Ralph H. Johnson Veterans Affairs Medical Center, Charleston, SC 29401, USA.
⁴Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.
⁵Department of Biochemistry and Molecular Medicine, Institute for Research in Immunology and Cancer, University of Montreal, Montreal, Quebec H3C IJ4, Canada.
⁶Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
⁷Department of Medicine, Research Institute of the McGill University Health Center, McGill University, Montreal, Quebec H4A 3J1, Canada.
⁸Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, CNRS, Université de Tours, 37380 Nouzilly, France.
⁹Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.
¹⁰Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA.
¹¹Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA.

↵*Corresponding author. Email: lefko001{at}receptor-biol.duke.edu

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Science Signaling 25 Sep 2018:
Vol. 11, Issue 549, eaat7650
DOI: 10.1126/scisignal.aat7650

Louis M. Luttrell

Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA.Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA.Research Service of the Ralph H. Johnson Veterans Affairs Medical Center, Charleston, SC 29401, USA.

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Jialu Wang

Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.

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Bianca Plouffe

Department of Biochemistry and Molecular Medicine, Institute for Research in Immunology and Cancer, University of Montreal, Montreal, Quebec H3C IJ4, Canada.

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ORCID record for Bianca Plouffe

Jeffrey S. Smith

Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.

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ORCID record for Jeffrey S. Smith

Lama Yamani

Department of Medicine, Research Institute of the McGill University Health Center, McGill University, Montreal, Quebec H4A 3J1, Canada.

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Suneet Kaur

Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.

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Pierre-Yves Jean-Charles

Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.

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Christophe Gauthier

Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, CNRS, Université de Tours, 37380 Nouzilly, France.

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Mi-Hye Lee

Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA.

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Biswaranjan Pani

Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.

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Jihee Kim

Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.

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Seungkirl Ahn

Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.

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Sudarshan Rajagopal

Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.

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Eric Reiter

Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, CNRS, Université de Tours, 37380 Nouzilly, France.

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Michel Bouvier

Department of Biochemistry and Molecular Medicine, Institute for Research in Immunology and Cancer, University of Montreal, Montreal, Quebec H3C IJ4, Canada.

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Sudha K. Shenoy

Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.

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Stéphane A. Laporte

Department of Medicine, Research Institute of the McGill University Health Center, McGill University, Montreal, Quebec H4A 3J1, Canada.

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Howard A. Rockman

Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA.

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Robert J. Lefkowitz

Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA.

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For correspondence: lefko001@receptor-biol.duke.edu

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vol. 11 no. 549

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https://doi.org/10.1126/scisignal.aat7650

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Received April 2, 2018
Accepted September 7, 2018
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Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works http://www.sciencemag.org/about/science-licenses-journal-article-reuseThis is an article distributed under the terms of the Science Journals Default License.

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Manifold roles of β-arrestins in GPCR signaling elucidated with siRNA and CRISPR/Cas9

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