Research ResourceBIOSENSORS Design and evaluation of engineered protein biosensors for live-cell imaging of EGFR phosphorylation Karthik Tiruthani1,*, Adam Mischler1,*, Shoeb Ahmed2,*, Jessica Mahinthakumar1, Jason M. Haugh1,†, and Balaji M. Rao1,†1Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA.2Department of Chemical Engineering, Bangladesh University of Engineering and Technology, Dhaka-1000, Bangladesh.↵†Corresponding author. Email: jmhaugh{at}ncsu.edu (J.M.H.); bmrao{at}ncsu.edu (B.M.R.)↵* These authors contributed equally to this work. See allHide authors and affiliations Science Signaling 04 Jun 2019:Vol. 12, Issue 584, eaap7584DOI: 10.1126/scisignal.aap7584 Karthik TiruthaniDepartment of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA.Find this author on Google Scholar Find this author on PubMed Search for this author on this site ORCID record for Karthik Tiruthani Adam MischlerDepartment of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA.Find this author on Google Scholar Find this author on PubMed Search for this author on this site ORCID record for Adam Mischler Shoeb AhmedDepartment of Chemical Engineering, Bangladesh University of Engineering and Technology, Dhaka-1000, Bangladesh.Find this author on Google Scholar Find this author on PubMed Search for this author on this site ORCID record for Shoeb Ahmed Jessica MahinthakumarDepartment of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA.Find this author on Google Scholar Find this author on PubMed Search for this author on this site ORCID record for Jessica Mahinthakumar Jason M. HaughDepartment of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA.Find this author on Google Scholar Find this author on PubMed Search for this author on this site ORCID record for Jason M. Haugh For correspondence: jmhaugh@ncsu.edu bmrao@ncsu.edu Balaji M. RaoDepartment of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA.Find this author on Google Scholar Find this author on PubMed Search for this author on this site ORCID record for Balaji M. Rao For correspondence: jmhaugh@ncsu.edu bmrao@ncsu.edu Article Figures & Data Info & Metrics eLetters PDF Article Information vol. 12 no. 584 DOI: https://doi.org/10.1126/scisignal.aap7584 PubMed: 31164479 Published By: Science Signaling Print ISSN: 1945-0877 Online ISSN: 1937-9145 History: Received August 23, 2017Accepted May 6, 2019 . Copyright & Usage: Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works http://www.sciencemag.org/about/science-licenses-journal-article-reuseThis is an article distributed under the terms of the Science Journals Default License. Author Information Altmetric Article usage Article lifetimeLast 6 monthsThis monthArticle usage: May 2019 to December 2019 AbstractFullPdf May 2019141 Jun 20194688825306 Jul 20191787329 Aug 20191085826 Sep 2019934834 Oct 20191034625 Nov 2019833721 Dec 20192289 View Full Text
Design and evaluation of engineered protein biosensors for live-cell imaging of EGFR phosphorylation By Karthik Tiruthani, Adam Mischler, Shoeb Ahmed, Jessica Mahinthakumar, Jason M. Haugh, Balaji M. Rao Science Signaling04 Jun 2019 Engineered biosensors accurately report the kinetics of EGFR activation. Supplementary Materials
Design and evaluation of engineered protein biosensors for live-cell imaging of EGFR phosphorylation By Karthik Tiruthani, Adam Mischler, Shoeb Ahmed, Jessica Mahinthakumar, Jason M. Haugh, Balaji M. Rao Science Signaling04 Jun 2019 Engineered biosensors accurately report the kinetics of EGFR activation. Supplementary Materials