Contents
28 January 2020
Vol 13, Issue 616
Vol 13, Issue 616
Focus
- Fragile epitopes—Antibody’s guess is as good as yours
Critical research antibodies need systematic validation (Frohner et al. and Schüchner et al., in 28 January 2020 issue).
Research Resources
- Antibodies recognizing the C terminus of PP2A catalytic subunit are unsuitable for evaluating PP2A activity and holoenzyme composition
C-terminal PP2A antibodies widely used to measure PP2A activity do not immunoprecipitate major PP2A holoenzyme classes.
- The Myc tag monoclonal antibody 9E10 displays highly variable epitope recognition dependent on neighboring sequence context
Recognition of Myc-tagged proteins by the widely used antibody 9E10 varies depending on sequences adjacent to the tag.
Editors' Choice
- Plant chemokine mimics
Human immune cells migrate in response to plant chemokine orthologs that bind to and activate the chemokine receptor CXCR4.
About The Cover
Online Cover This week features Research Resources by Frohner et al. and Schüchner et al. that highlight the importance of systematic validation of antibodies that are used for research (see also the associated Focus). The image shows an artist's depiction of an antibody. [Image: Kateryna Kon/Shutterstock]
