Editors' ChoiceMethodology

Genome-Wide DNA Arrays Elucidate Pathways

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Science's STKE  08 Feb 2000:
Vol. 2000, Issue 18, pp. tw9
DOI: 10.1126/stke.2000.18.tw9

Roberts et al. used DNA microarray analysis to observe global patterns of gene expression in yeast cells associated with alterations in signaling through the pheromone response pathway. Pheromone causes yeast cells to arrest in the G1 phase of the cell cycle and form mating projections. Pheromone receptors activate a mitogen-activated protein (MAP) kinase pathway that leads to alterations in gene expression. The results show the global patterns of gene regulation mediated by this pathway and related pathways that use MAP kinase signaling modules for other biological responses. They find that partial activation of the pheromone response pathway produces a gene expression pattern reminiscent of that in cells undergoing haploid invasive growth. Such regulation was previously thought to be under the control of a distinct MAP kinase pathway. Data from 383 highly regulated genes over 46 experimental conditions begin to show the predictive power of such large data sets when presented as a two-dimensional hierarchical matrix.

Roberts, C.J., Nelson, B., Marton, M.J., Stoughton, R., Meyer, M.R., Bennett, H.A., He, Y.D., Dai, H., Walker, W.L., Hughes, T.R., Tyers, M., Boone, C., Friend, S.H. (2000) Signaling and circuitry of multiple MAPK pathways revealed by a matrix of global gene expression profiles. Science 287: 873-880. [Abstract] [Full Text]

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