Editors' ChoiceImmunology

Dynamic PKD Movement

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Science's STKE  20 Jun 2000:
Vol. 2000, Issue 37, pp. tw9
DOI: 10.1126/stke.2000.37.tw9

Signals that are received by a cell surface receptor must be transmitted to the cell interior. Thus, the subcellular localization of relevant signaling molecules is critical for understanding how a signal transduction pathway disseminates information from the cell surface. Matthews et al. show that when the antigen receptors of live B cells or mast cells are activated, protein kinase D (PKD), a serine-threonine kinase located in the cytosol of these cells, is transiently recruited to the plasma membrane. PKD subsequently returns to the cytosol where it remains active for several hours. Translocation to the membrane was visualized by tagging PKD with green fluorescent protein. Membrane association was dependent on the diacylglycerol-binding domain of PKD. The authors propose that there are two stages of PKD regulation by antigen receptors--immediate activation, where PKD operates at the membrane, and a sustained response in which PKD functions in the cytosol.

Matthews, S.A., Iglesias, T., Rozengurt, E., and Cantrell, D. (2000) Spatial and temporal regulation of protein kinase D (PKD). EMBO J. 19: 2935-2945. [Abstract] [Full Text]

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